Human CL-P1/COLEC12 Antibody

Catalog # Availability Size / Price Qty
MAB26901
MAB26901-SP
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Human CL-P1/COLEC12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CL‑P1/COLEC12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CL-P1 is observed.
Source
Monoclonal Rat IgG1 Clone # 388305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CL‑P1/COLEC12 isoform 1
Leu57-Leu742
Accession # Q5KU26
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CL-P1/COLEC12

Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). With two exceptions, all collectins are secreted. CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo‑orosomucoids associated with the Lewisx epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full‑length CL-P1 (6). Human CL-P1 shares 93% aa sequence identity with mouse CL-P1 over the entire extracellular region, and 87% aa identity within each species CRD (5, 9). Human CL-P1 is expressed in vascular endothelial cells (5). CL-P1 may play a role in bacterial recognition or as a scavenger receptor for desialylated glycoproteins.

References
  1. van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.
  2. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
  3. Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
  4. Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
  5. Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
  6. Nakamura,K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
  7. Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
  8. Yoshida, T. et al. (2003) J. Biochem. 133:271.
  9. Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
Long Name
Collectin Placenta 1
Entrez Gene IDs
81035 (Human); 140792 (Mouse); 361289 (Rat)
Alternate Names
CL-P1; CLP1hCL-P1; COLEC12; collectin placenta 1; Collectin placenta protein 1; collectin sub-family member 12; collectin-12; NSR2; Nurse cell scavenger receptor 2; SCARA4; SCARA4NSR2; Scavenger receptor class A member 4; scavenger receptor class A, member 4; SRCL Type I; SRCLScavenger receptor with C-type lectin

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