Human Choline Acetyltransferase/ChAT Biotinylated Antibody

Catalog #: BAF3447
1 Citation Datasheet / COA / SDS

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BAF3447

All Choline Acetyltransferase/ChAT Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Choline Acetyltransferase/ChAT Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Choline Acetyltransferase/ChAT in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Choline Acetyltransferase/ChAT Isoform R
Ala2-Pro630
Accession # NP_066266

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Choline Acetyltransferase/ChAT Isoform R

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Choline Acetyltransferase/ChAT

ChAT catalyzes the production of the neurotransmitter acetylcholine (Ach), which is required for cholinergic neuron communication. It serves as a marker for functional cholinergic neurons in the central and peripheral nervous systems. At least six ChAT mRNAs encoding 69 kDa, 82 kDa and 74 kDa ChAT proteins have been identified. Compared to the 82 kDa form, 69 kDa ChAT lacks the N-terminal 118 amino acid extension containing a nuclear localization signal. As a result the 69 kDa ChAT is primarily localized to the cytoplasm. Human 69 kDa ChAT shares 86% amino acid sequence identity with the mouse or rat ChAT.

Entrez Gene IDs

1103 (Human); 12647 (Mouse); 290567 (Rat)

Alternate Names

acetyl CoA:choline O-acetyltransferase; CHAT; CHOACTase; Choline acetylase; Choline Acetyltransferase; choline O-acetyltransferase; CMS1A; CMS1A2; EC 2.3.1; EC 2.3.1.6

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human Choline Acetyltransferase/ChAT Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1