Human CETP Antibody

Catalog # Availability Size / Price Qty
MAB10042-100
MAB10042-SP
CETP in Human Liver.
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Human CETP Antibody Summary

Specificity
Detects human CETP in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 995234
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CETP
Cys18-Ser493
Accession # P11597
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CETP antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

CETP in Human Liver. CETP was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CETP Monoclonal Antibody (Catalog # MAB10042) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CETP

Cholesteryl Ester Transfer Protein (CETP), also known as plasma lipid transfer protein, is 493 amino acids (aa) secreted plasma protein involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles.  CETP allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL.

Long Name
Cholesteryl Ester Transfer Protein
Entrez Gene IDs
1071 (Human)
Alternate Names
BPIFF; CETP; cholesteryl ester transfer protein; cholesteryl ester transfer protein, plasma; HDLCQ10; Lipid Transfer Protein I

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Human CETP Antibody
By Anonymous on 05/30/2022
Application: IHC Sample Tested: Brain tissue Species: Human