Home / Cerberus 1 / Human Cerberus 1 Antibody

Human Cerberus 1 Antibody

Catalog #: AF1075
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1075
AF1075-SP
Detection of Human Cerberus 1 by Western Blot.
1 Image
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cerberus 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human DAN, recombinant mouse Gremlin, and recombinant chicken Caronte is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cerberus 1
Thy18-Ala267
Accession # O95813
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cerberus 1 antibody by Western Blot. View Larger

Detection of Human Cerberus 1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cerberus 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cerberus 1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerberus 1

Cerberus 1 functions during development by binding to and antagonizing some members of the bone morphogenetic protein (BMP) family. Cerberus 1 is alternatively known as cerberus-related 1 and is a homolog of Xenopus cerberus.

Entrez Gene IDs
9350 (Human); 12622 (Mouse)
Alternate Names
CER1; cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily); Cerberus 1; cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis); cerberus; cerberus-related 1; Cerberus-related protein; DAN domain family member 4; DAND4; MGC119894; MGC119895; MGC96951

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Cerberus 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm
    Authors: Mahaddalkar PU, Scheibner K, Pfluger S et al.
    Nature Biotechnology
  2. A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre
    Authors: Matteo A. Molè, Tim H. H. Coorens, Marta N. Shahbazi, Antonia Weberling, Bailey A. T. Weatherbee, Carlos W. Gantner et al.
    Nature Communications
  3. Secreted Cerberus1 as a Marker for Quantification of Definitive Endoderm Differentiation of the Pluripotent Stem Cells
    Authors: Hidefumi Iwashita, Nobuaki Shiraki, Daisuke Sakano, Takashi Ikegami, Masanobu Shiga, Kazuhiko Kume et al.
    PLoS ONE
  4. Distinct pathways drive anterior hypoblast specification in the implanting human embryo
    Authors: Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M;
    Nature cell biology
    Species: Human, Mouse
    Sample Types: Embryo
    Applications: Immunohistochemistry
  5. 3D-cultured blastoids model human embryogenesis from pre-implantation to early gastrulation stages
    Authors: Karvas, RM;Zemke, JE;Ali, SS;Upton, E;Sane, E;Fischer, LA;Dong, C;Park, KM;Wang, F;Park, K;Hao, S;Chew, B;Meyer, B;Zhou, C;Dietmann, S;Theunissen, TW;
    Cell stem cell
    Species: Human
    Sample Types: Protein
    Applications: Immunocytochemistry
  6. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm
    Authors: Mahaddalkar PU, Scheibner K, Pfluger S et al.
    Nature Biotechnology

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Cerberus 1 Antibody

There are currently no reviews for this product. Be the first to review Human Cerberus 1 Antibody and earn rewards!

Have you used Human Cerberus 1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review