Human CDCP1 Biotinylated Antibody

Catalog #: BAF2666 Datasheet / COA / SDS

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BAF2666

All CDCP1 Antibodies

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Human CDCP1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CDCP1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CDCP1 isoform 1
Phe30-Leu666
Accession # NP_073753

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CDCP1

Flow Cytometry

2.5 µg/10⁶ cells

SW480 human colorectal adenocarcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDCP1

CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 amino acid (aa) extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Sre kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa sequence identity with that of the mouse protein.

Long Name

CUB Domain Containing Protein 1

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Alternate Names

CD318 antigen; CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; SIMA135TRASK; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases