Home / CD68/SR-D1 / Human CD68/SR-D1 Antibody

Human CD68/SR-D1 Antibody

Catalog #: MAB2040
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2040
MAB2040-SP
Detection of CD68 in Human Tonsil via Multiplex Immunofluorescence staining on COMET™​
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD68/SR-D1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD68/SR‑D1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 298813
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD68/SR‑D1
Asn22-Ile320
Accession # AAB25811
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
Dual RNAscope ISH-IHC
5 µg/mL
See below
Multiplex Immunofluorescence
20 µg/mL
Paraffin embedded tissue sections of Human Tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Multiplex Immunofluorescence View Larger

Detection of CD68 in Human Tonsil via Multiplex Immunofluorescence staining on COMET™​ CD68 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD68 Monoclonal Antibody (Catalog # MAB2040) at 20 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Immunohistochemistry CD68/SR-D1 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

CD68/SR‑D1 in Human Tonsil. CD68/SR-D1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD68/SR-D1 Monoclonal Antibody (Catalog # MAB2040) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Dual RNAscope ISH-IHC Compatible CD68/SR-D1 antibody in Human Tonsil Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

CD68/SR-D1 in Human Tonsil Using Dual RNAscope®ISH and IHC. CD68/SR-D1 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 560591) followed by immunohistochemistry using R&D Systems Mouse Anti-Human CD68/SR-D1 Monoclonal Antibody (Catalog# MAB2040) at 5ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001). Tissue was stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (Catalog # 322500). Specific staining was localized to cytoplasm in lymphocytes.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD68/SR-D1

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.

Entrez Gene IDs
968 (Human); 12514 (Mouse); 287435 (Rat)
Alternate Names
CD_antigen: CD68; CD68 antigen; CD68 molecule; CD68; DKFZp686M18236; gp110; LAMP4; macrophage antigen CD68; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1

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Citations for Human CD68/SR-D1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005-06-01;46(6):659-67.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. AAV2-Mediated Transduction of the Mouse Retina After Optic Nerve Injury
    Authors: RW Nickells, HM Schmitt, ME Maes, CL Schlamp
    Invest. Ophthalmol. Vis. Sci., 2017-12-01;58(14):6091-6104.
  3. Proliferation and differentiation of direct co-culture of bone marrow mesenchymal stem cells and pigmented cells from the ciliary margin
    Authors: Li Y, He X, Li J et al.
    Mol Med Rep

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