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Human CD63 Antibody

Catalog #: MAB50481 1 Review Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB50481-100
MAB50481-SP
Detection of CD63 in Human PBMC by Flow Cytometry.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD63 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD63 in direct ELISAs.
Source
Monoclonal Rabbit IgG Clone # 2585C
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human PBMCs fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012); PBMC monocytes (surface stain)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CD63 antibody in Human PBMC antibody by Flow Cytometry. View Larger

Detection of CD63 in Human PBMC by Flow Cytometry. Human PBMC were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50481, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Immunocytochemistry CD63 antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

CD63 in A431 Human Cell Line. CD63 was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50481) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow (Cell Surface) View Larger

Detection of CD63 in PBMC monocytes by Flow Cytometry PBMC monocytes were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50481, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.

Entrez Gene IDs
967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)
Alternate Names
CD_antigen: CD63; CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30

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Human CD63 Antibody
By Anonymous on 12/13/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Endothelial cells Species: Human