Human CD48/SLAMF2 Antibody

Catalog #: AF3644 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3644
AF3644-SP

Detection of CD48/SLAMF2 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

1 Image

All CD48/SLAMF2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD48/SLAMF2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD48/SLAMF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse CD48 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD48/SLAMF2
Gln27-Ser220
Accession # P09326.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CD48/SLAMF2 (Catalog # 3644-CD)

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD48/SLAMF2 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Goat Anti-Human CD48/SLAMF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3644, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD48/SLAMF2

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules (1-3). The human CD48 cDNA encodes a 243 amino acid (aa) precursor that includes a 26 aa signal sequence, a 194 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide (4). A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients (5). Human CD48 shares approximately 50% aa sequence identity with mouse and rat CD48. It shares 20%‑26% aa sequence identity with comparable regions of human CD2 family members 2B4, CD2, BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors (4,6). Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC (7). CD2, 2B4, and heparan sulfate function as CD48 ligands (8-10). CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4 (8,11). CD48‑CD2 interactions promote T cell activation and class switching to IgG_2a in B cells (8,12). High affinity
CD48‑2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation (7,11,13,14). In mouse, CD48‑2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor (13). CD48‑2B4 mediated inhibition of NK cell activity is distinct from MHC I-restricted mechanisms (15). CD48 expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and nonexpressing cells can be targets of NK cell or CTL-mediated lysis (13,16).

References

Assarsson, E. et al. (2005) J. Immunol. 175:2045.
Bhat, R. et al. (2006) J. Leukocyte Biol. 79:417.
Loertscher, R. and P. Lavery (2002) Transpl. Immunol. 9:93.
Wong, Y.W. et al. (1990) J. Exp. Med. 171:2115.
Smith, G.M. et al. (1997) J. Clin. Immunol. 17:502.
Keil, M.J. et al. (2005) Cell 121:1109.
Morandi, B. et al. (2005) J. Immunol. 175:3690.
Kato, K. et al. (1992) J. Exp. Med. 176:1241.
Latchman, Y. et al. (1998) J. Immunol. 161:5809.
Ianelli, C.J. et al. (1998) J. Biol. Chem. 273:23367.
Messmer, B. et al. (2006) J. Immunol. 176:4646.
Gao, N. et al. (2005) J. Immunol. 174:4113.
Lee, K-M. et al. (2003) J. Immunol. 170:4881.
Lee, K-M. et al. (2006) Blood 107:3181.
McNerney, M.E. et al. (2005) Blood 106:1337.
Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.

Long Name

SLAM Family Member 2

Entrez Gene IDs

962 (Human); 12506 (Mouse); 245962 (Rat); 102134608 (Cynomolgus Monkey)

Alternate Names

BCM1 surface antigen; BCM1; BCM1Leukocyte antigen MEM-102; BLAST; BLAST1; BLAST1TCT.1; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); CD48 antigen; CD48 molecule; CD48; hCD48; mCD48; MEM-102; SLAMF2