Human CD44 v6 APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD44 v6 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD44 v6 APC-conjugated Monoclonal Antibody (Catalog # FAB3660A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD44
CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1-3). The standard or hematopoietic form, CD44H, does not include the variable segments (1-3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83% and 79% aa sequence identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).
- Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
- Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
- Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
- Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
- Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
- Nakamura, H. et al. (2004) Cancer Res. 64:876.
- Murakami, D. et al. (2003) Oncogene 22:1511.
- Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
- Fox, S.B. et al. (1994) Cancer Res. 54:4539.
Product Datasheets
Citations for Human CD44 v6 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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CNA Profiling of Single CTCs in Locally Advanced Esophageal Cancer Patients during Therapy Highlights Unexplored Molecular Pathways
Authors: G Gallerani, T Rossi, M Valgiusti, D Angeli, P Fici, S De Fanti, E Bandini, C Cocchi, GL Frassineti, M Bonafè, F Fabbri
Cancers, 2021-12-19;13(24):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Adipose stem cell niche reprograms the colorectal cancer stem cell metastatic machinery
Authors: S Di Franco, P Bianca, DS Sardina, A Turdo, M Gaggianesi, V Veschi, A Nicotra, LR Mangiapane, M Lo Iacono, I Pillitteri, S van Hooff, F Martorana, G Motta, E Gulotta, VL Lentini, E Martorana, ME Fiori, S Vieni, MR Bongiorno, G Giannone, D Giuffrida, L Memeo, L Colarossi, M Mare, P Vigneri, M Todaro, R De Maria, JP Medema, G Stassi
Nature Communications, 2021-08-18;12(1):5006.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Nobiletin and Xanthohumol Sensitize Colorectal Cancer Stem Cells to Standard Chemotherapy
Authors: A Turdo, A Glaviano, G Pepe, F Calapà, S Raimondo, ME Fiori, D Carbone, MG Basilicata, V Di Sarno, C Ostacolo, B Parrino, S Cascioferr, C Pecoraro, S Di Franco, D Bellavia, M Gaggianesi, V Veschi, M Lo Iacono, G Ganduscio, VD Pantina, LR Mangiapane, MR Bongiorno, R Alessandro, M Todaro, R De Maria, P Diana, P Campiglia, G Stassi
Cancers, 2021-08-04;13(16):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CHK1 inhibitor sensitizes resistant colorectal cancer stem cells to nortopsentin
Authors: Simone Di Franco, Barbara Parrino, Miriam Gaggianesi, Vincenzo Davide Pantina, Paola Bianca, Annalisa Nicotra et al.
iScience
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Pharmacological targeting of the novel &beta-catenin chromatin-associated kinase p38&alpha in colorectal cancer stem cell tumorspheres and organoids
Authors: M Lepore Sig, V Grossi, S Di Franco, G Forte, V Disciglio, C Fasano, P Sanese, K De Marco, FC Susca, LR Mangiapane, A Nicotra, G Di Carlo, F Dituri, G Giannelli, G Ingravallo, G Canettieri, G Stassi, C Simone
Cell Death & Disease, 2021-03-25;12(4):316.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
ROS and Lipid Droplet accumulation induced by high glucose exposure in healthy colon and Colorectal Cancer Stem Cells
Authors: Luca Tirinato, Francesca Pagliari, Simone Di Franco, Elisa Sogne, Maria Grazia Marafioti, Jeanette Jansen et al.
Genes & Diseases
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Expression of CD44v6-Containing Isoforms Influences Cisplatin Response in Gastric Cancer Cells
Authors: C Pereira, D Ferreira, N Mendes, PL Granja, GM Almeida, C Oliveira
Cancers (Basel), 2020-04-02;12(4):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant
Authors: V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi
Oncogene, 2019-10-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma
Mediators Inflamm, 2016-08-28;2016(0):9343460.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
Cell Stem Cell, 2014-03-06;14(3):342-56.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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