Human CD39/ENTPD1 Antibody

Catalog #: MAB43973 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB43973-100
MAB43973-SP

All CD39/ENTPD1 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD39/ENTPD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD39/ENTPD1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 498411

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD39/ENTPD1
Thr38-Val478
Accession # P49961

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human CD39/ENTPD1 Monoclonal Antibody (Catalog # MAB43972).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. rhNTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-1 ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). NTPDase-1 hydrolyzes the beta - and gamma phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (4). NTPDase-1 is involved in the processes of thrombo regulation and vascular inflammation (5). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (6).

References

Maliszewski, C.R. et al. (1994) J. Immunol. 153:3574.
Rowe, M. et al. (1982) Int. J. Cancer 29:373.
Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 1

Entrez Gene IDs

953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)

Alternate Names

ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1