Human CD35 Antibody

Catalog # Availability Size / Price Qty
MAB5748
MAB5748-SP
Detection of CD35 in Human PBMCs by Flow Cytometry.
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Human CD35 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD35 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat CSMD1, recombinant human (rh) CSMD2, rhCSMD3, rhHABP1/C1QBP, or rhComplement Factor H is observed.
Source
Monoclonal Mouse IgG1 Clone # 594708
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD35
Gln42-Asp1971 (His1208Arg)
Accession # CAA68755
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD35 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD35 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes (PBMC) were stained with Human CD35 Monoclonal Antibody (Catalog # MAB5748) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # MAB002 and IC002P, respectively).

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD35

CD35, also known as complement receptor 1 (CR1), is a 220-300 kDa N-glycosylated member of the RCA (regulators of complement activation) family of proteins. CD35 binds and internalizes particles and immune complexes that are opsonized with MBL or complement components C3b, C3i, C4b, or C1q. CD35 additionally protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases. The extracellular domain (ECD) of human CD35 contains 30 tandem SCR/SUSHI repeats. A soluble form of the CD35 ECD circulates in the serum. A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35. Cell surface CD35 is widely expressed on hematopoietic cells.

Entrez Gene IDs
1378 (Human)
Alternate Names
C3BR; CD35; complement component (3b/4b) receptor 1 (Knops blood group); CR1; KN

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