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Human CD3 epsilon Antibody

Catalog #: MAB100
69 Citations 4 Reviews Datasheet / COA / SDS
Clone UCHT1 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB100-SP
MAB100-100
MAB100-500
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CD3 epsilon in Human PBMCs.
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Citations (69)
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Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD3 epsilon Antibody Summary

Species Reactivity
Human
Specificity
Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Source
Monoclonal Mouse IgG1 Clone # UCHT1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human thymocytes (1)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation
McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York
 
CyTOF-reported
Behbehani, G.K. et al. (2015) Cancer Discov. 5: 988. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
T Cell Stimulation
This antibody can be used to activate T cells when immobilized at 1-10 μg/mL (100 μL/well).
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD3e antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD3 epsilon in Human PBMCs. CD3e was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot View Larger

Detection of Human CD3 epsilon by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), and THP‑1 human acute monocytic leukemia cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry Detection of CD3e antibody in Human Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of Mouse CD3 epsilon by Flow Cytometry View Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow Cytometry View Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow Cytometry View Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow Cytometry View Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow Cytometry View Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow Cytometry View Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry View Larger

Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry View Larger

Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry View Larger

Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry View Larger

Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry View Larger

Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry View Larger

Detection of Mouse Human CD3 epsilon Antibody by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References
  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Entrez Gene IDs
916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names
CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Citations for Human CD3 epsilon Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

69 Citations: Showing 1 - 10
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  1. Comparison study of different indoleamine-2,3 dioxygenase inhibitors from the perspective of pharmacodynamic effects
    Authors: Xue Jiang, Xiaopeng Li, Shuang Zheng, Guangying Du, Jinbo Ma, Liming Zhang et al.
    Int J Immunopathol Pharmacol
  2. Duokines: a novel class of dual-acting co-stimulatory molecules acting in cis or trans
    Authors: Sina Fellermeier-Kopf, Friederike Gieseke, Ugur Sahin, Dafne Müller, Klaus Pfizenmaier, Roland E. Kontermann
    OncoImmunology
  3. Role of natural killer cells in liver transplantation treatment of liver cancer
    Authors: Wenbin Ji, Jin Chen, Yuche Mi, Guiliang Wang, Xinjiang Xu, Weizheng Wang
    Experimental and Therapeutic Medicine
  4. Cytokine-induced killer cell therapy for modulating regulatory T cells in patients with non-small cell lung cancer
    Authors: Baodan Yu, Junli Wang, Chen He, Wei Wang, Jianli Tang, Runhui Zheng et al.
    Experimental and Therapeutic Medicine
  5. Multiple Homozygous Variants in the STING-Encoding TMEM173 Gene in HIV Long-Term Nonprogressors
    Authors: SK Nissen, JG Pedersen, M Helleberg, K Kjaer, K Thavachelv, N Obel, M Tolstrup, MR Jakobsen, TH Mogensen
    J. Immunol., 2018-04-09;0(0):.
  6. Reduced circulating interleukin 35 is associated with enhanced peripheral T cell function in primary biliary cholangitis
    Authors: Siqi Liu, Qian Zhang, Mengyao Zhang, Xuejing Zhong, Wudong Wang, Lishuang Wang et al.
    Bosnian Journal of Basic Medical Sciences
  7. The homodimer interfaces of costimulatory receptors B7 and CD28 control their engagement and pro-inflammatory signaling
    Authors: Popugailo, A;Rotfogel, Z;Levy, M;Turgeman, O;Hillman, D;Levy, R;Arad, G;Shpilka, T;Kaempfer, R;
    Journal of biomedical science
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Anti-Inflammatory Effects of 1,25(OH)2D/Calcitriol in T Cell Immunity: Does Sex Make a Difference?
    Authors: D Peruzzu, ML Dupuis, M Pierdomini, K Fecchi, MC Gagliardi, E Ortona, MT Pagano
    International Journal of Molecular Sciences, 2022-08-15;23(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  9. Overexpression of ascitic interleukin-35 induces CD8+ T cell exhaustion in liver cirrhotic patients with spontaneous bacterial peritonitis
    Authors: L Yang, S Liu, Q Zhang, S Jia, C Qiu, Z Jin
    International immunopharmacology, 2022-03-26;108(0):108729.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Increased IL-6 expression precedes reliable viral detection in the rhesus macaque brain during acute SIV infection
    Authors: RM Gopalakris, M Aid, NB Mercado, C Davis, S Malik, E Geiger, V Varner, R Jones, SE Bosinger, C Piedra-Mor, AJ Martinot, DH Barouch, RK Reeves, CS Tan
    JCI Insight, 2021-10-22;6(20):.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC
  11. Glycosaminoglycans bind human IL-27 and regulate its activity
    Authors: MC Cavé, S Maillard, K Hildenbran, C Mamelonet, MJ Feige, O Devergne
    Eur. J. Immunol., 2020-07-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  12. Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2)
    Authors: J Huetter, U Gritzan, I Gutcher, WD Doecke, MV Luetke-Eve, S Golfier, HG Roider, AL Frisk, J Hunter, A Pow, A Drake, Z Levine, O Levy, M Azulay, I Barbiro, G Cojocaru, I Vaknin, B Kreft, L Roese
    Cancer Immunol Res, 2020-04-20;8(7):895-911.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay, T Cell Activation
  13. Ex vivo culture of lesional psoriasis skin for pharmacological testing
    Authors: ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato
    J. Dermatol. Sci., 2019-12-27;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Tissue Culture
  14. Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models
    Authors: JC Chamcheu, S Esnault, VM Adhami, AL Noll, S Banang-Mbe, T Roy, SS Singh, S Huang, KG Kousoulas, H Mukhtar
    Cells, 2019-09-15;8(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  15. Effect of morphine and a low dose of ketamine on the T cells of patients with refractory cancer pain in vitro
    Authors: NB Zhou, KG Wang, ZJ Fu
    Oncol Lett, 2019-08-16;18(4):4230-4236.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  16. SLAMF6 clustering is required to augment T cell activation
    Authors: MA Dragovich, K Adam, M Strazza, AS Tocheva, M Peled, A Mor
    PLoS ONE, 2019-06-14;14(6):e0218109.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  17. IL-15-based trifunctional antibody-fusion proteins with costimulatory TNF-superfamily ligands in the single-chain format for cancer immunotherapy
    Authors: N Beha, M Harder, S Ring, RE Kontermann, D Müller
    Mol. Cancer Ther., 2019-04-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. 89Zr-atezolizumab imaging as a non-invasive approach to assess clinical response to PD-L1 blockade in cancer
    Authors: F Bensch, EL van der Ve, MN Lub-de Hoo, A Jorritsma-, R Boellaard, IC Kok, SF Oosting, CP Schröder, TJN Hiltermann, AJ van der We, HJM Groen, TC Kwee, SG Elias, JA Gietema, SS Bohorquez, A de Crespig, SP Williams, C Mancao, AH Brouwers, BM Fine, EGE de Vries
    Nat. Med., 2018-11-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
    Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
    Sci Rep, 2017-07-26;7(1):6607.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  20. Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue
    Authors: C Caër, C Rouault, T Le Roy, C Poitou, J Aron-Wisne, A Torcivia, JC Bichet, K Clément, M Guerre-Mil, S André
    Sci Rep, 2017-06-07;7(1):3000.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis
    Authors: S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio
    Arthritis Res. Ther., 2017-05-16;19(1):97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  22. PLC?1 regulates SDF-1?-induced lymphocyte adhesion and migration to sites of inflammation
    Authors: M Strazza, I Azoulay-Al, M Peled, AV Smrcka, EY Skolnik, S Srivastava, A Mor
    Proc. Natl. Acad. Sci. U.S.A, 2017-02-17;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function
    Authors: C Tao, H Shao, W Zhang, H Bo, F Wu, H Shen, S Huang
    Mol Med Rep, 2017-02-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts
    Authors: Elena Neumann
    Matrix Biol., 2016-11-22;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  25. Human trophoblast cells induced MDSCs from peripheral blood CD14(+) myelomonocytic cells via elevated levels of CCL2
    Authors: Xun Qu
    Cell. Mol. Immunol., 2015-06-01;13(5):615-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines.
    Authors: Huang W, Lu C, Wu Y, Ouyang S, Chen Y
    J Exp Clin Cancer Res, 2015-05-21;34(0):54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  27. PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation.
    Authors: Patsoukis N, Bardhan K, Chatterjee P, Sari D, Liu B, Bell L, Karoly E, Freeman G, Petkova V, Seth P, Li L, Boussiotis V
    Nat Commun, 2015-03-26;6(0):6692.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  28. Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation.
    Authors: Moore C, Cui Q, Warsi N, Durafourt B, Zorko N, Owen D, Antel J, Bar-Or A
    J Immunol, 2014-12-10;194(2):761-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  29. 1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients.
    Authors: Van Belle T, Vanherwegen A, Feyaerts D, De Clercq P, Verstuyf A, Korf H, Gysemans C, Mathieu C
    PLoS ONE, 2014-10-03;9(10):e109194.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.
    Authors: Fan, Miaomiao, Zhang, Yijun, Huang, Zhuoqion, Liu, Jun, Guo, Xuemin, Zhang, Hui, Luo, Haihua
    PLoS ONE, 2014-09-24;9(9):e108253.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  31. Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation.
    Authors: Zhu L, Huang Z, Stalesen R, Hansson G, Li N
    J Thromb Haemost, 2014-06-27;12(7):1156-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in gastric and colorectal cancer patients.
    Authors: Gao D, Li C, Xie X, Zhao P, Wei X, Sun W, Liu H, Alexandrou A, Jones J, Zhao R, Li J
    PLoS ONE, 2014-04-03;9(4):e93886.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma.
    Authors: Ruan M, Thorn K, Liu S, Li S, Yang W, Zhang C, Zhang C
    Int J Oncol, 2014-03-21;44(6):2103-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  34. CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
    Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
    Cell Stem Cell, 2014-03-06;14(3):342-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  35. IkappaB kinase epsilon targets interferon regulatory factor 1 in activated T lymphocytes.
    Authors: Sgarbanti M, Marsili G, Remoli A, Stellacci E, Mai A, Rotili D, Perrotti E, Acchioni C, Orsatti R, Iraci N, Ferrari M, Borsetti A, Hiscott J, Battistini A
    Mol Cell Biol, 2014-01-06;34(6):1054-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  36. Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
    Authors: Renand A, Milpied P, Rossignol J, Bruneau J, Lemonnier F, Dussiot M, Coulon S, Hermine O
    PLoS ONE, 2013-12-30;8(12):e85589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  37. A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1.
    Authors: Cevikbas F, Wang X, Akiyama T, Kempkes C, Savinko T, Antal A, Kukova G, Buhl T, Ikoma A, Buddenkotte J, Soumelis V, Feld M, Alenius H, Dillon S, Carstens E, Homey B, Basbaum A, Steinhoff M
    J Allergy Clin Immunol, 2013-12-25;133(2):448-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  38. Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases.
    Authors: Wang, Feng, Xu, Zhiwen, Zhou, Jie, Lo, Wing-Sze, Lau, Ching-Fu, Nangle, Leslie A, Yang, Xiang-Le, Zhang, Mingjie, Schimmel, Paul
    J Biol Chem, 2013-09-03;288(41):29223-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  39. B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis.
    Authors: Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S
    J. Immunol., 2011-03-02;186(0):4518.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  40. Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
    Authors: Eikawa S, Ohue Y, Kitaoka K
    J. Immunol., 2010-11-03;185(11):6734-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  41. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
    Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
    FASEB J., 2010-09-08;25(1):409-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010-03-31;184(9):4880-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  43. Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
    Authors: Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K
    Genes Immun., 2009-10-01;10(8):702-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  44. Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
    Authors: Ni Z, Walcheck B
    Clin. Immunol., 2009-08-08;133(2):257-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  45. Phase I therapeutic trial of the HIV-1 Tat protein and long term follow-up.
    Authors: Longo O, Tripiciano A, Fiorelli V, Bellino S, Scoglio A, Collacchi B, Alvarez MJ, Francavilla V, Arancio A, Paniccia G, Lazzarin A, Tambussi G, Din CT, Visintini R, Narciso P, Antinori A, D'Offizi G, Giulianelli M, Carta M, Di Carlo A, Palamara G, Giuliani M, Laguardia ME, Monini P, Magnani M, Ensoli F, Ensoli B
    Vaccine, 2009-02-07;27(25):3306-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  46. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
    Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
    Blood, 2009-02-06;113(24):6120-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  47. Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells.
    Authors: Hirosako S, Goto E, Fujii K, Tsumori K, Hirata N, Tsumura S, Kamohara H, Kohrogi H
    Clin. Exp. Immunol., 2008-11-26;155(2):266-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  48. Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
    Authors: Tran CN, Thacker SG, Louie DM, Oliver J, White PT, Endres JL, Urquhart AG, Chung KC, Fox DA
    J. Immunol., 2008-03-01;180(5):2989-98.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  49. Preferential migration of T regulatory cells induced by IL-16.
    Authors: McFadden C, Morgan R, Rahangdale S, Green D, Yamasaki H, Center D, Cruikshank W
    J. Immunol., 2007-11-15;179(10):6439-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  50. Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
    Authors: Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U
    J. Immunol., 2007-09-15;179(6):4239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  51. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007-04-01;178(7):4184-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  52. Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis.
    Authors: Mamoni RL, Blotta MH
    Cytokine, 2006-10-11;35(3):207-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  53. A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood.
    Authors: Markovic SN, Nevala WK, Uhl CB, Celis E, McKean DJ
    Clin. Exp. Immunol., 2006-09-01;145(3):438-47.
    Species: Human
    Sample Types:
    Applications: Binding Assay
  54. B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease.
    Authors: Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux NY, Goncalves RB, Valverde P, Dibart S, Li YP, Miranda LA, Ernst CW, Izumi Y, Taubman MA
    Am. J. Pathol., 2006-09-01;169(3):987-98.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-Fr, Immunoprecipitation
  55. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005-06-01;46(6):659-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  56. Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay.
    Authors: Ogilvie RL, Abelson M, Hau HH, Vlasova I, Blackshear PJ, Bohjanen PR
    J. Immunol., 2005-01-15;174(2):953-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  57. Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
    Authors: Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY
    J. Orthop. Res., 2005-01-01;23(1):203-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  58. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004-08-01;21(2):241-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  59. Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor.
    Authors: Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM
    Biochem. J., 2004-06-01;380(0):425-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  60. HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis.
    Authors: Janardhan A, Swigut T, Hill B, Myers MP, Skowronski J
    PLoS Biol., 2004-01-20;2(1):E6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  61. In vitro differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
    Authors: Takahashi R, Mizukawa Y, Yamazaki Y, Hayakawa K, Hayakawa J, Kudo A, Shiohara T
    J. Immunol., 2003-12-01;171(11):5769-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  62. NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
    Authors: Han MK, Kim JS, Park BH, Kim JR, Hwang BY, Lee HY, Song EK, Yoo WH
    J. Leukoc. Biol., 2003-04-01;73(4):525-9.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  63. TOM1L is involved in a novel signaling pathway important for the IL-2 production in Jurkat T cells stimulated by CD3/CD28 co-ligation
    Authors: Ahmed Elmarghani, Hanan Abuabaid, Peter Kjellen
    Mediators of Inflammation
  64. Fc-based Duokines: dual-acting costimulatory molecules comprising TNFSF ligands in the single-chain format fused to a heterodimerizing Fc (scDk-Fc)
    Authors: Nadine Aschmoneit, Katharina Kocher, Martin Siegemund, Martina S. Lutz, Lennart Kühl, Oliver Seifert et al.
    OncoImmunology
  65. Interleukin-25 initiates Th2 differentiation of human CD4(+) T cells and influences expression of its own receptor
    Authors: Graeme Bredo, Jessica Storie, Nami Shrestha Palikhe, Courtney Davidson, Alexis Adams, Harissios Vliagoftis et al.
    Immunity, Inflammation and Disease
  66. RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures
    Authors: Dapeng Li, Todd Bradley, Derek W. Cain, Isabela Pedroza-Pacheco, Maria Aggelakopoulou, Robert Parks et al.
    ImmunoHorizons
  67. Influence of antigen density and immunosuppressive factors on tumor-targeted costimulation with antibody-fusion proteins and bispecific antibody-mediated T cell response
    Authors: Sabrina Sapski, Nadine Beha, Roland E. Kontermann, Dafne Müller
    Cancer Immunology, Immunotherapy
  68. NaHCO3 enhances the antitumor activities of cytokine-induced killer cells against hepatocellular carcinoma HepG2 cells
    Authors: Ya Hong Yuan, Chun Fang Zhou, Jiang Yuan, Li Liu, Xing Rong Guo, Xiao Li Wang et al.
    Oncology Letters

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Human CD3 epsilon Antibody
By Danlee Enzler on 04/18/2018
Application: IHC Sample Tested: Spleen tissue Species: Mouse
Human CD3 epsilon Antibody
By Leslie Priddy on 04/13/2018
Application: IHC Sample Tested: Tumor cell lyastes Species: Human
Human CD3 epsilon Antibody
By Anonymous on 03/21/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human
Human CD3 epsilon Antibody
By Anonymous on 10/25/2017
Application: ELISA Sample Tested: Human recombinant antibody Species: Human