Human CD27/TNFRSF7 Biotinylated Antibody

Catalog #: BAF382 Datasheet / COA / SDS

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BAF382

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Human CD27/TNFRSF7 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD27 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) sTNF RI and rhsTNF RII (under reducing conditions) is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD27
Thr21-Ile192
Accession # P26842

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CD27/TNFRSF7 Fc Chimera (Catalog # 382-CD)

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood lymphocytes

Immunohistochemistry

3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CD27/TNFRSF7 in Human Tonsil. CD27/TNFRSF7 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CD27/TNFRSF7 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF382) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27/TNFRSF7

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4⁺ compartment, it is preferentially expressed on CD45RA⁺ cells. In contrast, it is preferentially expressed on CD45RO⁺ cells in the CD8⁺ compartment. CD27 also appeaars to be a potential marker for memory B cells. It exists as both a disulfide-linked dimer on the cell surface and as a soluble protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 20 aa signal, a 173 aa extracellular domain, a 20 aa transmembrane domain, and a 47 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and a small subset of activated T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses.

References

Camerini, D. et al. (1991) J. Immunol. 147:3165.
Loenen, W.A. et al. (1992) J. Immunol. 149:3937.
Lens, S.M.A. et al. (1998) Sem. Immunol. 10:491.

Entrez Gene IDs

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Alternate Names

CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7