Human CD25/IL-2R alpha Antibody

Catalog #: MAB10202 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10202-100
MAB10202-SP

Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody.

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Human CD25/IL-2R alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD25/IL-2R alpha in ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1037230

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human CD25/IL-2R alpha
Glu22-Cys213
Accession # P01589

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND₅₀) is typically 0.2-2.0 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. Recombinant Human IL-2 (202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10202). The ND₅₀ is typically 0.2-2.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2R alpha), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit (gamma c). Human IL-2R alpha cDNA encodes a 213 amino acid (aa) precursor with a 21 aa signal peptide and a 192 aa extracellular region. The ECD of Human IL-2R alpha shares a 59% amino acid sequence identity with the ECD of mouse and rat IL-2R alpha, respectively. IL‑2R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). IL-2R beta (CD122) and gamma c (IL-2R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (4, 5). By itself, IL-2R alpha binds IL-2 with low affinity. IL-2R alpha makes no contacts with IL-2R beta or gamma c, and only minor changes are observed in the IL-2 structure in response to receptor binding. These findings support the principal role of IL-2R alpha to deliver IL-2 to the signaling complex and act as regulator of signal transduction (6, 7). A soluble form of IL‑2R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL‑2R alpha are found in some cancers and immune disorders (10). IL-2R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2R alpha is also required for the development of CD4+CD25+ Treg which suppresses autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11-13).

References

Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Kovanen, P.E. and Leonard, W.J. (2004) Immunol. Rev. 202:67.
Bluestone, J.A. and Tang, Q. (2005) Curr. Opin. Immunol. 17:638.
Hatakeyama, M. et al. (1989) Science 244:551.
Takeshita, T. et al. (1992) Science 257:379.
Stauber, D. et al. (2006) Proc. Natl. Acad. Sci. U. S. A. 103:2788.
Wang, X. et al. (2005) Science 310:1159.
Wagner, D.K. et al. (1986) J. Immunol. 137:592.
Schulz, O. et al. (1998) J. Exp. Med. 187:271.
Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
Willerford, D.M. et al. (1995) Immunity 3:521.
Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Long Name

Interleukin 2 Receptor alpha

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Alternate Names

CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR