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Human CD163 Antibody

Catalog #: AF1607
15 Citations 1 Review Datasheet / COA / SDS
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AF1607
AF1607-SP
Detection of Human CD163 by Western Blot.
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Citations (15)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD163 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD163 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD163 (R&D Systems, Catalog # 1607-CD)
Gly41-Ser1045
Accession # CAA80543
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of Human Kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD163 antibody by Western Blot. View Larger

Detection of Human CD163 by Western Blot. Western blot shows lysates of human thymus tissue, human liver tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for CD163 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human CD163 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human CD163 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD163 at approximately 179 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Immunohistochemistry View Larger

Detection of CD163 in Human Kidney. CD163 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD163

CD163, previously called M130 or p155, is a 130-160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1-3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a
39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86%, and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7 - 10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL-10, IL-6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb-Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13-15).

References
  1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
  2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
  4. Kristiansen, M. et al. (2001) Nature 409:198.
  5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
  6. Moller, H.J. et al. (2002) Blood 99:378.
  7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
  8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
  9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
  10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
  11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
  13. Schaer, D.J. et al. (2006) Blood 107:373.
  14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  15. Fabriek, B.O. et al. (2007) Blood 109:5223.
Entrez Gene IDs
9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names
CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163

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Citations for Human CD163 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. A Single Amino Acid Substitution in Porcine Reproductive and Respiratory Syndrome Virus Glycoprotein 2 Significantly Impairs Its Infectivity in Macrophages
    Authors: J Chaudhari, RA Leme, K Durazo-Mar, S Sillman, AM Workman, HLX Vu
    Viruses, 2022-12-18;14(12):.
    Species: Chlorocebus pygerythrus, Porcine
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b' Is Not Required for In Vitro Virus Replication
    Authors: Y Cai, S Yu, Y Fang, L Bollinger, Y Li, M Lauck, EN Postnikova, S Mazur, RF Johnson, CL Finch, SR Radoshitzk, G Palacios, TC Friedrich, TL Goldberg, DH O'Connor, PB Jahrling, JH Kuhn
    Viruses, 2021-04-07;13(4):.
    Species: Primate - C. aethiops
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Isolation and characterization of a new population of nasal surface macrophages and their susceptibility to PRRSV-1 subtype 1 (LV) and subtype 3 (Lena)
    Authors: D Oh, J Xie, N Vanderheij, HJ Nauwynck
    Vet. Res., 2020-02-24;51(1):21.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC
  4. Accumulation of stabilin-1 positive macrophages in the early stage of gastric cancer is associated with short cumulative survival
    Authors: SP Yin, Y Gao, XS Xie, DD Xu, V Riabov, WD Du
    Oncol Lett, 2020-01-16;19(3):2404-2412.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IF
  5. Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells
    Authors: J Xie, I Christiaen, B Yang, I Trus, B Devriendt, T Cui, R Wei, HJ Nauwynck
    Vet. Res., 2018-07-18;49(1):67.
    Species: Human, Porcine
    Sample Types: Whole Cells
    Applications: ICC
  6. Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model
    Oncotarget, 2016-05-24;7(21):31097-110.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Simian hemorrhagic fever virus cell entry is dependent on CD163 and uses a clathrin-mediated endocytosis-like pathway.
    Authors: Cai Y, Postnikova E, Bernbaum J, Yu S, Mazur S, Deiuliis N, Radoshitzky S, Lackemeyer M, McCluskey A, Robinson P, Haucke V, Wahl-Jensen V, Bailey A, Lauck M, Friedrich T, O'Connor D, Goldberg T, Jahrling P, Kuhn J
    J Virol, 2014-10-29;89(1):844-56.
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Whole Cells
    Applications: Neutralization
  8. CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms.
    Authors: Subramanian K, Du R, Tan N, Ho B, Ding J
    J Immunol, 2013-04-15;190(10):5267-78.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Bioassay, Flow Cytometry, ICC, Western Blot
  9. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
    Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
    J Immunol, 2012-08-31;189(7):3508-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.
    Authors: De Baere MI, Van Gorp H, Delputte PL
    Vet. Res., 2012-05-25;43(1):47.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus.
    Authors: Van Gorp H, Van Breedam W, Van Doorsselaere J, Delputte PL, Nauwynck HJ
    J. Virol., 2009-12-23;84(6):3101-5.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Western Blot
  12. Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus.
    Authors: Van Gorp H, Van Breedam W, Delputte PL, Nauwynck HJ
    J. Gen. Virol., 2008-12-01;89(0):2943-53.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  13. Availability of activated CD4+ T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys.
    Authors: Klatt NR, Villinger F, Bostik P, Gordon SN, Pereira L, Engram JC, Mayne A, Dunham RM, Lawson B, Ratcliffe SJ, Sodora DL, Else J, Reimann K, Staprans SI, Haase AT, Estes JD, Silvestri G, Ansari AA
    J. Clin. Invest., 2008-06-01;118(6):2039-49.
    Species: Primate - Cercocebus atys (Sooty Mangabey)
    Sample Types: Whole Tissue
    Applications: IHC
  14. CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses.
    Authors: Calvert JG, Slade DE, Shields SL, Jolie R, Mannan RM, Ankenbauer RG, Welch SK
    J. Virol., 2007-05-09;81(14):7371-9.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Heterogeneous macrophages contribute to the pathology of disc herniation induced radiculopathy
    Authors: Jin L, Xiao L, Ding M Et al.
    The spine journal : official journal of the North American Spine Society

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Human CD163 Antibody
By Anonymous on 11/06/2017
Application: WB Sample Tested: Cancer cell lysates Species: Human