Human CD160 Antibody

Catalog #: AF6177 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6177
AF6177-SP

Detection of Human CD160 by Western Blot.

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Human CD160 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD160 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse CD160 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD160
Ile27-Ser159
Accession # O95971

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CD160 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Human CD160 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD160 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD160

CD160 (also BY55) is a 27 kDa member of Ig Superfamily of molecules. It is expressed on select hematopoietic cell types, including CD56^dim CD16⁺ cytotoxic NK cells, CD8⁺ CD28^- effector T cells, gamma δ T cells, and restricted CD4⁺ T cells. It is a receptor for HLA-C molecules, and its engagement induces CD160⁺ NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program. Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27-181) (SwissProt #:O95971). It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one V‑type Ig-like domain (aa 27-122). CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180Leu181, and shows a 20 aa TM segment between aa 163-182. The TM form appears to have a splice variant that lacks aa 25-133. Over aa 27-159, human CD160 shares only 62% aa sequence identity with mouse CD160.

Entrez Gene IDs

11126 (Human); 54215 (Mouse); 502585 (Rat); 102117412 (Cynomolgus Monkey)

Alternate Names

BY55; BY55FLJ46513; CD160 antigenimmunoglobulin superfamily member; CD160 molecule; CD160; Natural killer cell receptor BY55; NK1; NK28