Human CD133 APC-conjugated Antibody

Catalog #: FAB11331A
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB11331A-025
FAB11331A-100

Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry.

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All CD133 Antibodies

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Citations (2)

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Human CD133 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD133. Stains human CD133 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 170411

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CD133
Accession # O43490

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry. NS0 mouse myeloma cell line transfected with either human CD133 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human CD133 APC‑conjugated Monoclonal Antibody (Catalog # FAB11331A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD133

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells.

Entrez Gene IDs

8842 (Human); 19126 (Mouse)

Alternate Names

AC133 Stargardt disease 4 (autosomal dominant); AC133; CD133 retinal 2; CD133; PROM1; prominin (mouse)-like 1; Prominin 1; PROML1

Product Datasheets

Product Datasheet

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Citations for Human CD133 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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High dose acetaminophen inhibits STAT3 and has free radical independent anti-cancer stem cell activity
Authors: Pavani Pingali, Y. Jeffrey Wu, Rio Boothello, Chetna Sharon, Howard Li, Srinivas Sistla et al.
Neoplasia
The FGF, TGF&beta and WNT axis Modulate Self-renewal of Human SIX2+ Urine Derived Renal Progenitor Cells
Authors: MS Rahman, W Wruck, LS Spitzhorn, L Nguyen, M Bohndorf, S Martins, F Asar, A Ncube, L Erichsen, N Graffmann, J Adjaye
Sci Rep, 2020-01-20;10(1):739.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry