Human CCR7 Antibody

Catalog #: MAB197R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB197R-100
MAB197R-SP

Detection of CCR7 in Human PBMCs by Flow Cytomtetry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR7.

Source

Recombinant Monoclonal Mouse IgG_2A Clone # 150503R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human CCR7 transfectants
Met1-Pro378
Accession # AAA58615

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 50 ng/mL Recombinant Human CCL19/MIP‑3 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of CCR7 antibody in Human PBMCs by Flow Cytomtetry.

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Detection of CCR7 in Human PBMCs by Flow Cytomtetry. Human peripheral blood mononuclear cells (PBMCs) were stained with PE-conjugated Mouse anti-Human CD4 Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197R) or (B) Mouse IgG_2Aisotype control antibody (Catalog # MAB003) followed by APC-conjugated anti-mouse IgG secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Neutralization

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Chemotaxis Induced by CCL19/MIP-3 beta and Neutral-ization by Human CCR7 Antibody. Recombinant Human CCL19/MIP-3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197R). The ND₅₀ is typically 1-5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Entrez Gene IDs

1236 (Human); 12775 (Mouse); 287673 (Rat)

Alternate Names

BLR2; BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7; CMKBR7chemokine (C-C) receptor 7; EBI1; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor