Human CCL26/Eotaxin-3 Antibody

Catalog #: MAB6533 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6533-100
MAB6533-SP

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL26/Eotaxin-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL26/Eotaxin-3 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 115009

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived human CCL26/Eotaxin-3
Thr24-Leu94
Accession # Q9Y258.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human CCL26/Eotaxin‑3 Monoclonal Antibody(Catalog # MAB6532).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CCL26/Eotaxin-3 DuoSet ELISA Kit (Catalog # DY346) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL26/Eotaxin-3

Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin-3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4.

Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. Recombinant Eotaxin-3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. Recombinant Eotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin-3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3-transfected cell line.

References

Gou, R.F. et al. (1999) Genomics 58:313.
Kitamura, M. et al. (1999) J. Biol. Chem. 274:27975.
Shinkai, A. et al. (1999) J. Immunol. 163:1602.

Entrez Gene IDs

10344 (Human)

Alternate Names

CC chemokine IMAC; C-C motif chemokine 26; CCL26; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin3; Eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4a; MIP-4alpha; SCYA26; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; Small-inducible cytokine A26; TSC-1Thymic stroma chemokine-1