Home / CCL13/MCP-4 / Human CCL13/MCP-4 Antibody

Human CCL13/MCP-4 Antibody

Catalog #: MAB327
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB327
MAB327-SP
Chemotaxis Induced by CCL13/MCP‑4 and Neutral-ization by Human CCL13/ MCP‑4 Antibody.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL13/MCP-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL13/MCP‑4 in ELISAs. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) CCL2 is observed and no significant cross-reactivity or interference with rhCCL5, 7, 8, 11, 24, recombinant mouse CCL2, 5, 7, 11 or 12 is observed.
Source
Monoclonal Mouse IgG1 Clone # 73506
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL13/MCP-4
Gln24-Thr98
Accession # Q99616.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
A549 human lung carcinoma cell line treated with recombinant human IL-1 beta, fixed with paraformaldehyde and permeabilized with saponin

Human CCL13/MCP-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL13/MCP‑4 Biotinylated Antibody (Catalog # BAF327)

Standard: Recombinant Human CCL13/MCP-4 Protein (Catalog # 327-P4)

Neutralization
Measured by its ability to neutralize CCL13/MCP‑4-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 2 µg/mL Recombinant Human CCL13/MCP‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL13/MCP‑4 and Neutral-ization by Human CCL13/ MCP‑4 Antibody. View Larger

Chemotaxis Induced by CCL13/MCP‑4 and Neutral-ization by Human CCL13/ MCP‑4 Antibody. Recombinant Human CCL13/ MCP-4 (Catalog # 327-P4) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL13/MCP-4 (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL13/ MCP-4 Monoclonal Antibody (Catalog # MAB327). The ND50 is typically 10-30 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL13/MCP-4

CCL13, also known as Monocyte Chemoattractant Protein-4 (MCP-4), is a CC chemokine that acts as a chemoattractant for monocytes, eosinophils and T cells and as an activator of basophils. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. The bioactivities of CCL13 are mediated by the CC chemokine receptors CCR-2 and CCR-3.

Entrez Gene IDs
6357 (Human)
Alternate Names
C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human CCL13/MCP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. The Global Phosphorylation Landscape of SARS-CoV-2 Infection
    Authors: Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al.
    Cell
  2. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Blister Fluid, Cell Culture Supernates
    Applications: ELISA Development, Neutralization

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human CCL13/MCP-4 Antibody

There are currently no reviews for this product. Be the first to review Human CCL13/MCP-4 Antibody and earn rewards!

Have you used Human CCL13/MCP-4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review