Human Carnosine Dipeptidase 1/CNDP1 Antibody

Catalog #: MAB2489 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2489
MAB2489-SP

Carnosine Dipeptidase 1/CNDP1 in Human Heart.

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Human Carnosine Dipeptidase 1/CNDP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carnosine Dipeptidase 1/CNDP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CNDP2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 352216

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carnosine Dipeptidase 1/CNDP1
Pro28-His507
Accession # Q96KN2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN)

Immunohistochemistry

8-25 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN), see our available Western blot detection antibodies

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Carnosine Dipeptidase 1/CNDP1 in Human Heart. Carnosine Dipeptidase 1/CNDP1 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Carnosine Dipeptidase 1/CNDP1 Monoclonal Antibody (Catalog # MAB2489) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry

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Carnosine Dipeptidase 1/CNDP1 in BG01V Human Embryonic Stem Cells. Carnosine Dipeptidase 1/CNDP1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neurons using Mouse Anti-Human Carnosine Dipeptidase 1/CNDP1 Monoclonal Antibody (Catalog # MAB2489) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carnosine Dipeptidase 1/CNDP1

The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta -Ala (carnosine) and Xaa = gamma -aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase.

References

Teufel, M. et al. (2004) J. Biol. Chem. 278:6521.
Bauer, K. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1022, Academic Press, San Diego.

Entrez Gene IDs

84735 (Human)

Alternate Names

beta-Ala-His dipeptidase; Carnosinase 1; carnosine dipeptidase 1 (metallopeptidase M20 family); Carnosine Dipeptidase 1; CN1MGC102737; CNDP dipeptidase 1; CNDP1; CPGL2; CPGL2MGC142072; EC 3.4.13.20; Glutamate carboxypeptidase-like protein 2; HsT2308; MGC10825; Serum carnosinase