Human Carbohydrate Sulfotransferase 15/CHST15 Antibody

Catalog #: AF3365
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3365
AF3365-SP

All Carbohydrate Sulfotransferase 15/CHST15 Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbohydrate Sulfotransferase 15/CHST15 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbohydrate Sulfotransferase 15/CHST15 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbohydrate Sulfotransferase 15/CHST15
Ser99-Thr561
Accession # Q7LFX5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Carbohydrate Sulfotransferase 15/CHST15 (Catalog # 3365-ST)

Flow Cytometry

2.5 µg/10⁶ cells

Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 15/CHST15

N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST, or, CHST15) is a type II transmembrane protein composed of a short N-terminal cytoplasmic domain and a long C-terminal luminal catalytic domain. GalNAc4S-6ST transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the 6-O of GalNAc-4S on chondroitin sulfate A (CS-A), as well as dermatan sulfate (1), generating the chondroitin sulfate E (CS-E) disaccharide unit GlcA beta 1-3GalNAc-4S,6S. CS-E binds with strong affinity to Midkine, a heparan sulfate-binding growth factor (2), playing an important regulatory role in differentiation and morphogenesis during embryonic development. In situ hybridization for the expression of GalNAc4S-6ST in the postnatal mouse brain showed a widespread expression of the transcript in the developing brain except at postnatal day 7 (3). For comparison, chondroitin sulfate 6-O sulfotransferase-1 (C6ST-1) encoded by the CHST3 gene, only recognizes and transfers sulfate to the 6-O of unsulfated GalNAc residues on chondroitin sulfate generating the chondroitin sulfate C (CS-C) disaccharide unit GlcA beta 1-3GalNAc-6S (4). The sequence of the human GalNAc4S-6ST was found to be the same as human B-cell RAG-associated protein (BRAG) (1).

References

Ohtake, S. et al. (2001) J. Biol. Chem. 276:43894.
Zou, P. et al. (2003) Glycobiology 13:35.
Purushothaman, A. et al. (2007) J. Biol. Chem. 282:19442.
Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10155.
Robbins, P.W. (1962) Methods in Enzymol. 5:964.
MacRae, I.J. et al. (2000) Biochemistry 39:1613.
Wu, Z.L. et al. (2002) FASEB J. 16:539.

Entrez Gene IDs

51363 (Human); 286974 (Rat)

Alternate Names

B cell RAG associated protein (GALNAC4S-6ST); B-cell RAG-associated gene protein; BRAG; BRAGKIAA0598RP11-47G11.1; carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15; Carbohydrate Sulfotransferase 15; CHST15; DKFZp781H1369; EC 2.8.2.33; GALNAC4S6ST; GalNAc4S-6ST; hBRAG; MGC34346; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human Carbohydrate Sulfotransferase 15/CHST15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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