Human CapG Antibody

Catalog #: AF7177 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7177
AF7177-SP

Detection of Human CapG by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CapG Antibody Summary

Species Reactivity

Human

Specificity

Detects human CapG in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CapG
Met1-Lys348
Accession # P40121

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CapG by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human CapG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CapG at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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CapG in U937 Human Cell Line. CapG was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-Human CapG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7177) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CapG

CapG ("Caps" barbed actin ends and resembles Gelsolin; also Macrophage capping protein/MCP and actin regulatory protein Cap-G) is a 40-42 kDa member of the Villin/Gelsolin family of proteins. It is expressed by macrophages/monocytes and endothelial cells, and is found in both cytoplasm and nucleus. It appears to be involved in cell motility, and is known to bind to (but not cleave) actin filament ends in a calcium-dependent manner. This regulates the growth of actin filaments. Human CapG is 348 amino acids (aa) in length. It contains an N-terminal gelsolin repeat (aa 27-75), followed by a NLS (aa 137-146), two consecutive gelsolin-like repeats (aa 148-188 and 261-307), and one utilized phosphorylation site at Ser337. There is at least one potential isoform variant that contains a deletion of aa 64-84. Full-length human CapG shares 91% aa sequence identity with mouse CapG.

Long Name

Capping Protein, Gelsolin-like

Entrez Gene IDs

822 (Human); 12332 (Mouse); 297339 (Rat)

Alternate Names

actin-regulatory protein CAP-G; AFCP; CapG; capping protein (actin filament), gelsolin-like; gelsolin-like capping protein; macrophage capping protein; macrophage-capping protein; MCPActin regulatory protein CAP-G