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Human C-Reactive Protein/CRP Antibody

Catalog #: MAB17074 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB17074-100
MAB17074-SP
Detection of Human C‑Reactive Protein/CRP by Western Blot.
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Human C-Reactive Protein/CRP Antibody Summary

Species Reactivity
Human
Specificity
Detects human C-Reactive Protein/CRP in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 987318
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human C‑Reactive Protein/CRP
Phe17-Pro224
Accession # P02741
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human C-Reactive Protein/CRP antibody by Western Blot. View Larger

Detection of Human C‑Reactive Protein/CRP by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human C-Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17074) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for C-Reactive Protein/CRP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C-Reactive Protein/CRP

CRP is a secreted protein that belongs to the Pentraxin family. It circulates as a non-covalent homopentamer. The CRP concentration in human plasma is highly increased during acute phase response to tissue injury or infection.

Entrez Gene IDs
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

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