Human BTNL8 Antibody Summary
Gln18-Lys238
Accession # Q6UX41-1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human BTNL8 by Western Blot. Western blot shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with human BTNL8 and eGFP. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTNL8 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of BTNL8 in HEK293 Human Cell Line Transfected with Human BTNL8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human BTNL8 and eGFP was stained with either (A) Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human BTNL8 by Simple WesternTM. Simple Western lane view shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with with human BTNL8 and eGFP, loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 66 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human BTNL8 by Simple WesternTM. Simple Western lane view shows lysates of human small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 60 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTNL8
Butyrophilin-like
8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative
regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I
transmembrane glycoprotein that contains a signal peptide followed by an
extracellular domain (ECD), a transmembrane region and a short cytoplasmic
domain (2). The ECD of human BTNL8 shares 88% sequence identity with the
ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and
BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3).
The complete immunological function of BTNL molecules is only beginning to
emerge. BTNL8 has been shown to be important in initiation of primary immune
responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and
colon cancer (4). BTNL8
are expressed in colon, lung, testis and neutrophils, and its expression is
significantly decreased in ulcerative colitis, colonic tumors as compared to
unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but
not activated T cells. In vitro,
BTNL8 co-stimulates T cell proliferation and cytokine production. In
vivo injections of BTNL8-Fc significantly increases
production of
Ag-specific IgG during the primary but not the secondary immune
response (3).
- Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
- Arnett, H.A. et al. (2009) Cytokine 46:370.
- Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
- Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.
Product Datasheets
Citation for Human BTNL8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease
Authors: Toufic Mayassi, Kristin Ladell, Herman Gudjonson, James E. McLaren, Dustin G. Shaw, Mai T. Tran et al.
Cell
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