Human BTN3A1/2/3 Antibody

Catalog #: AF7136 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7136
AF7136-SP

Detection of Human BTN3A1/2/3 by Western Blot.

1 Image

All BTN3A1/2/3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BTN3A1/2/3 Antibody Summary

Specificity

Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BTN3A2
Gln30-Trp248
Accession # P78410

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human BTN3A1/2/3 by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BTN3A1/2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7136) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BTN3A1/2/3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN3A1/2/3

BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region (aa 30-248) with one V-type Ig-like domain (aa 30-134), and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. One possesses an alternative start site 43 aa upstream of the standard site, a second contains a five aa substitution for aa 1-28, and a third shows a 24 aa substitution for aa 307-334. A rodent counterpart to BTN3A2 has not been reported. Human BTN3A1 (Butyrophilin subfamily 3 member A1; also BTF5) over aa 30-248 shares 95.4% aa identity with human NTN3A2.

Long Name

Butyrophilin, Subfamily 3, Member A1/2/3

Alternate Names

BT3.1;BTF5;BTN3.1;BTN3A1;CD277; BTN3A1/2/3