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Human Brachyury Antibody

Catalog #: MAB20851
9 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB20851-SP
MAB20851-100
Brachyury in BG01V Human Embryonic Stem Cells.
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Product Details
Citations (9)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Brachyury Antibody Summary

Species Reactivity
Human
Specificity
Detects human Brachyury in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1161B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human Brachyury
Met230-Met435
Accession # O15178
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Brachyury antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Brachyury in BG01V Human Embryonic Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Rabbit Anti-Human Brachyury Monoclonal Antibody (Catalog # MAB20851) at a 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brachyury

Brachyury is a 435 aa T-box family transcription factor.  Human Brachyury shares 90% and 91% aa identity with mouse and rat Brachyury, respectively.  It is required in the early determination and differentiation of mesoderm.  Additionally, expression of Brachyury has been shown to be upregulated in a number of cancers, and in some cases, correlates with increased aggressiveness.

Entrez Gene IDs
6862 (Human)
Alternate Names
brachyury protein; Brachyury; MGC104817; Protein T; SAVA; T brachyury (mouse) homolog; T brachyury homolog; T Brachyury Transcription Factor; T, brachyury homolog (mouse); T-Box Transcription Factor T; TBXT; TF; TFT

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Citations for Human Brachyury Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture models
    Authors: Hilansi Rawat, Jessica Kornherr, Dorota Zawada, Sara Bakhshiyeva, Christian Kupatt, Karl-Ludwig Laugwitz et al.
    Frontiers in Cell and Developmental Biology
  2. 7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome
    Authors: H Tomita, KM Hines, JM Herron, A Li, DW Baggett, L Xu
    Elife, 2022-09-16;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation
    Authors: AM Drozd, L Mariani, X Guo, V Goitea, NA Menezes, E Ferretti
    International Journal of Molecular Sciences, 2022-09-07;23(18):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  4. Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
    Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
    International Journal of Molecular Sciences, 2022-06-30;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. A Micropatterned Human‐Specific Neuroepithelial Tissue for Modeling Gene and Drug‐Induced Neurodevelopmental Defects
    Authors: Geetika Sahni, Shu‐Yung Chang, Jeremy Teo Choon Meng, Jerome Zu Yao Tan, Jean Jacques Clement Fatien, Carine Bonnard et al.
    Advanced Science
  6. Endoplasmic Reticulum Stress Activation in Alport Syndrome Varies Between Genotype and Cell Type
    Authors: Cong Wang, Shenghui Liang, Shijia Xing, Ke Xu, Huijie Xiao, Haiyue Deng et al.
    Frontiers in Genetics
  7. Combined PDGFR and HDAC Inhibition Overcomes PTEN Disruption in Chordoma.
    Authors: Lee D, Zhang Y, Kassam A, Park M, Gardner P, Prevedello D, Henry S, Horbinski C, Beumer J, Tawbi H, Williams B, Shaffrey M, Egorin M, Abounader R, Park D
    PLoS ONE, 2015-08-06;10(8):e0134426.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  8. Measles vector as a multiple genes delivery platform facilitating iPSC reprogramming
    Authors: Q Wang, A Vossen, Y Ikeda, P Devaux
    Gene Ther., 2019-02-04;0(0):.
  9. WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids
    Authors: A Yoney, F Etoc, A Ruzo, T Carroll, JJ Metzger, I Martyn, S Li, C Kirst, ED Siggia, AH Brivanlou
    Elife, 2018-10-12;7(0):.

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