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Human BMP-8 Antibody

Catalog #: AF1073
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1073
AF1073-SP
BMP‑8 in Human Osteo-sarcoma.
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Citations (2)
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Human BMP-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMP-8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BMP‑2, rhBMP-3, rhBMP-4, rhBMP-5, rhBMP-6 and rhBMP-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human BMP‑8a
Ala264-His402
Accession # AAP74559
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human BMP‑8
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry BMP-8 antibody in Human Osteo-sarcoma by Immunohistochemistry (IHC-P). View Larger

BMP‑8 in Human Osteo-sarcoma. BMP-8 was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using 15 µg/mL Goat Anti-Human BMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1073) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-8

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7 (OP-1) (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs (2). BMPs play roles in regulating growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal, epithelial, hematopoetic, and neuronal cells.

There exist two highly related and closely linked genes, designated BMP-8a and -8b in mice and humans. For humans, the protein products of these two genes share 98% amino acid (aa) sequence identity in their pro- and mature regions. However in the mouse, the two proteins share 89% and 76% aa sequence homology in their pro- and mature regions, respectively (3). Mature human BMP-8a shares 91% and 70% aa sequence identity with mouse BMP-8a and -8b, respectively. Human BMP‑8a is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RTPR) between aa residues 263 and 264 to release a 139 aa carboxy-terminal domain. Expression patterns of the BMP-8 genes indicate that they regulate aspects of cell proliferation and/or differentiation during spermatogenesis and formation of the placenta (3). BMP-8 is also highly expressed in osteosarcomas (4).

References
  1. Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
  2. Canalis, E. et al. (2003) Endocrine Rev. 24:218.
  3. Zhao, G-Q. et al. (1996) Mech. Dev. 57:159.
  4. Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.
Long Name
Bone Morphogenetic Protein 8
Entrez Gene IDs
353500 (Human); 12163 (Mouse)
Alternate Names
BMP8; BMP-8; BMP8A; BMP-8A; OP-2

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Citations for Human BMP-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Unveiling the transcriptomic landscape and the potential antagonist feedback mechanisms of TGF-beta superfamily signaling module in bone and osteoporosis
    Authors: Ying-Wen Wang, Wen-Yu Lin, Fang-Ju Wu, Ching-Wei Luo
    Cell Communication and Signaling
  2. The protective role of bone morphogenetic protein-8 in the glucocorticoid-induced apoptosis on bone cells.
    Authors: Kosa JP, Kis A, Bacsi K, Balla B, Nagy Z, Takacs I, Speer G, Lakatos P
    Bone, 2011-01-27;48(5):1052-7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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