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Human BMP-2/BMP-4 Antibody

Catalog #: MAB3552
15 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3552-SP
MAB3552-100
MAB3552-500
Alkaline Phosphatase Production Induced by BMP‑2 and Neutralization by Human BMP-2/BMP-4 Antibody.
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Citations (15)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-2/BMP-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMP-2 and human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant zebra fish (rzf) BMP-2 and rhBMP-4, 20%-40% cross-reactivity with rhBMP-3, -3b, -6, and no cross-reactivity with rhBMP-5, -7, -10, or -15 is observed.
Source
Monoclonal Mouse IgG1 Clone # 100230
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human BMP-2
Gln283-Arg396
Accession # P12643
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BMP-2 (Catalog # 355-BM)
Recombinant Human BMP-4 (Catalog # 314-BP)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize BMP‑2-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑2 and 2 µg/mL heparin. This antibody also neutralizes Human BMP-4 induced activity in the ATDC5 mouse chondrogenic cell line. The ND50 is approximately 3 μg/mL in the presence of 75 ng/mL of Recombinant Human BMP-4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Alkaline Phosphatase Production Induced by BMP‑2 and Neutralization by Human BMP-2/BMP-4 Antibody. View Larger

Alkaline Phosphatase Production Induced by BMP‑2 and Neutralization by Human BMP-2/BMP-4 Antibody. Recombinant Human BMP-2 (Catalog # 355-BM) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Human BMP-2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-2/BMP-4 Monoclonal Antibody (Catalog # MAB3552). The ND50 is typically 5-15 µg/mL in the presence of heparin (2 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2/BMP-4

Bone Morphogenetic Protein 2 (BMP-2) and BMP-4 are two of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor  beta (TGF-beta ) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs.

Long Name
Bone Morphogenetic Protein 2/4
Entrez Gene IDs
650 (Human); 12156 (Mouse); 29373 (Rat)
Alternate Names
BDA2; BMP-2; BMP-2/BMP-4; BMP2A; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2

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Citations for Human BMP-2/BMP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome
    Authors: Bersini S, Schulte R, Huang L et al.
    eLife
  2. Interplay of Oct4 with Sox2 and Sox17: a molecular switch from stem cell pluripotency to specifying a cardiac fate
    Authors: Sonia Stefanovic, Nesrine Abboud, Stéphanie Désilets, David Nury, Chad Cowan, Michel Pucéat
    Journal of Cell Biology
  3. Exposure to Uremic Serum Induces a Procalcific Phenotype in Human Mesenchymal Stem Cells
    Authors: Rafael Kramann, Simone K. Couson, Sabine Neuss, Uta Kunter, Manfred Bovi, Jörg Bornemann et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  4. Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides
    Authors: Paul T. Hamilton, Michelle S. Jansen, Sathya Ganesan, R. Edward Benson, Robin Hyde-DeRuyscher, Wayne F. Beyer et al.
    PLoS ONE
  5. Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells
    Authors: Bruce A. Boswell, Linda S. Musil
    Molecular Biology of the Cell
  6. Smad1/5 is required for erythropoietin-mediated suppression of hepcidin in mice
    Authors: CY Wang, AB Core, S Canali, KB Zumbrennen, S Ozer, L Umans, A Zwijsen, JL Babitt
    Blood, 2017-04-24;0(0):.
  7. The Hematopoietic TALE-Code Shows Normal Activity of IRX1 in Myeloid Progenitors and Reveals Ectopic Expression of IRX3 and IRX5 in Acute Myeloid Leukemia
    Authors: S Nagel, C Pommerenke, C Meyer, RAF MacLeod
    International Journal of Molecular Sciences, 2022-03-16;23(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses
    Authors: W Chen, SS Foo, E Hong, C Wu, WS Lee, SA Lee, D Evseenko, MEL Moreira, A García-Sas, G Cheng, K Nielsen-Sa, P Brasil, E Avvad-Port, JU Jung
    Nature Microbiology, 2021-01-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis.
    Authors: Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A
    PLoS ONE, 2015-06-25;10(6):e0131453.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Essential role of BMPs in FGF-induced secondary lens fiber differentiation.
    Authors: Boswell BA, Overbeek PA, Musil LS
    Dev. Biol., 2008-09-18;324(2):202-12.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Regulation of bone morphogenetic protein signalling in human pulmonary vascular development.
    Authors: Southwood M, Jeffery TK, Yang X, Upton PD, Hall SM, Atkinson C, Haworth SG, Stewart S, Reynolds PN, Long L, Trembath RC, Morrell NW
    J. Pathol., 2008-01-01;214(1):85-95.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Iron transferrin regulates hepcidin synthesis in primary hepatocyte culture through hemojuvelin and BMP2/4.
    Authors: Lin L, Valore EV, Nemeth E, Goodnough JB, Gabayan V, Ganz T
    Blood, 2007-05-31;110(6):2182-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia.
    Authors: Rydziel S, Stadmeyer L, Zanotti S, Durant D, Smerdel-Ramoya A, Canalis E
    J. Biol. Chem., 2007-05-10;282(27):19762-72.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  14. Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7.
    Authors: Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT
    J. Cell. Biochem., 2005-05-01;95(1):1-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  15. Bone morphogenetic protein 2 controls iron homeostasis in mice independent of Bmp6
    Authors: Susanna Canali, Chia-Yu Wang, Kimberly B. Zumbrennen-Bullough, Abraham Bayer, Jodie L. Babitt
    American Journal of Hematology

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Human BMP-2/BMP-4 Antibody
By Anonymous on 07/25/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Aorta tissue Species: Human