Human BMI-1 Antibody Summary
Asp96-Gly326
Accession # P35226
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![BMI‑1 antibody in K562 Human Cell Line by Immunocytochemistry (ICC). BMI‑1 antibody in K562 Human Cell Line by Immunocytochemistry (ICC).](https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_AF3334_Immunocytochemistry_6492.jpg)
BMI‑1 in K562 Human Cell Line. BMI-1 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using 10 µg/mL Goat Anti-Human BMI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3334) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMI-1
BMI-1 (B cell-specific Moloney-MLV Integration site 1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of large multimeric complex termed PRC1 that inhibits target gene transcription loss of BMI-1 function and precludes stem cells from self-replicating. The amino acid sequence of human BMI-1 is 99%, 97%, 99%, and 99% identical to that of bovine, mouse, feline, and canine BMI-1, respectively.
Product Datasheets
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