Human BMI-1 Antibody

Catalog # Availability Size / Price Qty
MAB33341
MAB33341-SP
Detection of Human BMI‑1 by Western Blot.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human BMI-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMI-1 in direct ELISAs  and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 384515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human BMI-1
Asp96-Gly326
Accession # P35226
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BMI‑1 antibody by Western Blot. View Larger

Detection of Human BMI‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.1 µg/mL Mouse Anti-Human BMI-1 Monoclonal Antibody (Catalog # MAB33341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for BMI-1 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Chromatin Immunoprecipitation (ChIP) Detection of BMI-1-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of BMI‑1-regulated Genes by Chromatin Immunoprecipitation. HeLa human cervical epithelial carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. BMI-1/DNA complexes were immunoprecipitated using 5 µg Mouse Anti-Human BMI-1 Monoclonal Antibody (Catalog # MAB33341) or control antibody (Catalog # MAB003) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody (Catalog # BAF007). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thehoxc13promoter was detected by standard PCR.

Intracellular Staining by Flow Cytometry Detection of BMI‑1 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of BMI‑1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human BMI-1 Monoclonal Antibody (Catalog # MAB33341, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMI-1

BMI-1 (B cell-specific Moloney-MLV integration site #1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain (aa 17-56), an NLS (aa 81-95) and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 shares 99%, 97%, 99% and 99% aa sequence identity with bovine, mouse, feline and canine BMI-1, respectively.

Long Name
B Lymphoma Mo-MLV Insertion Region 1
Entrez Gene IDs
648 (Human); 12151 (Mouse)
Alternate Names
B lymphoma Mo-MLV insertion region 1 homolog (mouse); B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1; BMI-1; FLVI2/BMI1; MGC12685; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; RING finger protein 51; RNF51; RNF51Polycomb group RING finger protein 4

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Citation for Human BMI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. BMI1 reprogrammes histone acetylation and enhances c-fos pathway via directly binding to Zmym3 in malignant myeloid progression.
    Authors: Shen H, Chen Z, Ding X, Qi X, Cen J, Wang Y, Yao L, Chen Y
    J Cell Mol Med, 2014-02-27;18(6):1004-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot

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Human BMI-1 Antibody
By Anonymous on 03/22/2017
Application: WB Sample Tested: MCF-7 human breast cancer cell line Species: Human

MCF-7 cell lysate probed with 1:2,500 Mouse anti-Human BMI-1 Antibody. Buffer: 5% BSA in PBS. Secondary Ab: anti-Mouse IgG 1:5,000.