Human BLIMP1/PRDM1 Antibody Summary
Lys667-Cys789
Accession # O75626
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human BLIMP1/PRDM1 by Western Blot. Western blot shows lysates of U266 human myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for BLIMP1/PRDM1 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human BLIMP1/PRDM1 by Simple WesternTM. Simple Western lane view shows lysates of U266 human myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for BLIMP1/PRDM1 at approximately 114 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.
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Detection of BLIMP1/PRDM1 in MM.1S cells by Flow Cytometry MM.1S cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BLIMP1/PRDM1
Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.
Product Datasheets
Citations for Human BLIMP1/PRDM1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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Induced Pluripotent Stem Cells Derived From Two Idiopathic Azoospermia Patients Display Compromised Differentiation Potential for Primordial Germ Cell Fate
Authors: Fang Fang, Zili Li, Qian Zhao, Zhen Ye, Xiuli Gu, Feng Pan et al.
Frontiers in Cell and Developmental Biology
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GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program
Authors: Yoji Kojima, Chika Yamashiro, Yusuke Murase, Yukihiro Yabuta, Ikuhiro Okamoto, Chizuru Iwatani et al.
Life Science Alliance
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Massively parallel knock-in engineering of human T cells
Authors: X Dai, JJ Park, Y Du, Z Na, SZ Lam, RD Chow, PA Renauer, J Gu, S Xin, Z Chu, C Liao, P Clark, H Zhao, S Slavoff, S Chen
Nature Biotechnology, 2023-01-26;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A Notch/STAT3-driven Blimp-1/c-Maf-dependent molecular switch induces IL-10 expression in human CD4+ T cells and is defective in Crohn�s disease patients
Authors: J Ahlers, A Mantei, L Lozza, M Stäber, F Heinrich, P Bacher, T Hohnstein, L Menzel, SG Yüz, D Alvarez-Si, AR Bickenbach, C Weidinger, N Mockel-Ten, AA Kühl, B Siegmund, J Maul, C Neumann, A Scheffold
Mucosal Immunology, 2022-02-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation
Authors: D Chen, W Liu, J Zimmerman, WA Pastor, R Kim, L Hosohama, J Ho, M Aslanyan, JJ Gell, SE Jacobsen, AT Clark
Cell Rep, 2018-12-26;25(13):3591-3602.e5.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Effect of plasma-activated medium on the decrease of tumorigenic population in lymphoma
Authors: N Wada, JI Ikeda, H Tanaka, H Sakakita, M Hori, Y Ikehara, E Morii
Pathol. Res. Pract., 2017-04-20;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
IMiD immunomodulatory compounds block C/EBP{beta} translation through eIF4E down-regulation resulting in inhibition of MM.
Authors: Li S, Pal R, Monaghan SA, Schafer P, Ouyang H, Mapara M, Galson DL, Lentzsch S
Blood, 2011-03-09;117(19):5157-65.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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