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Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Catalog #: MAB6664
1 Citation Datasheet / COA / SDS
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MAB6664
MAB6664-SP
Detection of Human beta -1,4-Glucuronyltransferase 1/B4GAT1 by Western Blot.
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Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta -1,4-Glucuronyltransferase 1/B4GAT1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B3GNT2, rhB3GNT6, or rhB4GalT1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 724057
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human  beta -1,4-Glucuronyltransferase 1/B4GAT1 Antibody
Asp43-Cys415
Accession # O43505
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human beta -1,4-Glucuronyltransferase 1/B4GAT1 antibody by Western Blot. View Larger

Detection of Human beta -1,4-Glucuronyltransferase 1/B4GAT1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta -1,4-Glucuronyltransferase 1/B4GAT1 Monoclonal Antibody (Catalog # MAB6664) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta -1,4-Glucuronyltransferase 1/B4GAT1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,4-Glucuronyltransferase 1/B4GAT1

B3GNT1 ( beta -1,3-N-acetylglucosaminyltransferase 1; also iGnT) is a 50 kD member of the b3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451 amino acid (aa) type II transmembrane protein. It contains an eight aa N-terminal cytoplasmic segment, an extended 28 aa transmembrane region (aa 9-36), and a 379 aa luminal domain (aa 37-415). Over aa 37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.

Long Name
beta-1,4-Glucuronyltransferase 1
Entrez Gene IDs
11041 (Human); 108902 (Mouse); 293667 (Rat)
Alternate Names
B3GNT1; B3GN-T1; B4GAT1; beta-1,3-N-acetylglucosaminyltransferase bGnT-6; BETA3GNTI; EC 2.4.1.149; i-beta-1,3-N-acetylglucosaminyltransferase; iGAT; iGNT; MDDGA13; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1B3GNT6; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6

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Citation for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation.
    Authors: Voss M, Kunzel U, Higel F, Kuhn P, Colombo A, Fukumori A, Haug-Kroper M, Klier B, Grammer G, Seidl A, Schroder B, Obst R, Steiner H, Lichtenthaler S, Haass C, Fluhrer R
    EMBO J, 2014-10-29;33(24):2890-905.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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