Human beta -1,3-Glucuronyltransferase 1/B3GAT1 Antibody

Catalog #: MAB85601 Datasheet / COA / SDS

Human B3GAT1 MAb (Clone 1021236)

Catalog #	Availability	Size / Price	Qty
MAB85601-100
MAB85601-SP

beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 in SH‑SY5Y Human Cell Line.

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Human beta -1,3-Glucuronyltransferase 1/B3GAT1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1021236

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human beta ‑1,3‑Glucuronyltransferase 1/B3GAT1
His25-Ile334
Accession # Q9P2W7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed SH-SY5Y human neuroblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 in SH‑SY5Y Human Cell Line. beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line (positive) and A549 human lung carcinoma cell line (negative control) using Mouse Anti-Human beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB85601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips Protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO₃-3GlcA beta 1-3Gal beta 1-4GlcNAc (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3). The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

References

Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.
Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.
Kakuda, S. et al. (2005) Glycobiology 2:203.
Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.
Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase

Entrez Gene IDs

27087 (Human); 76898 (Mouse); 117108 (Rat)

Alternate Names

B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); Beta-1,3-glucuronyltransferase 1; CD57; EC 2.4.1.135; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GLCATP; GlcAT-P; GLCATPGLCUATP; GLCUATP; glcUAT-P; Glucuronosyltransferase P; HNK1; LEU7; NK1; NK-1; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase