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Human BCMA/TNFRSF17 Antibody

Catalog #: AF193
7 Citations Datasheet / COA / SDS
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AF193
AF193-SP
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Detection of BCMA/TNFRSF17 in U266 Human Cell Line by Flow Cytometry.
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Citations (7)
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Human BCMA/TNFRSF17 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BCMA in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhTACI, and recombinant mouse BCMA is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BCMA/TNFRSF17
Met1-Ala54
Accession # Q6PE46
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC)
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody(Catalog # MAB1932).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human BCMA/TNFRSF17 DuoSet ELISA Kit (Catalog # DY193) for convenient development of a sandwich ELISA.

 
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.3-1.2 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP) to immobilized Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC) coated at 1 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human BCMA/TNFRSF17 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human BCMA/TNFRSF17 Biotinylated Antibody (Catalog # BAF193)

Standard: Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF (Catalog # 193-BC)

Human/Primate BCMA/TNFRSF17 Sandwich Immunoassay

Recommended Concentration
Reagent
 

Use in combination with:

Standard: Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF (Catalog # 193-BC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of BCMA/TNFRSF17 antibody in U266 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of BCMA/TNFRSF17 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Goat Anti-Human BCMA/TNFRSF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF193, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCMA/TNFRSF17

BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References
  1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
  2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
  4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
  5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
B Cell Maturation Factor
Entrez Gene IDs
608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)
Alternate Names
B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17

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Citations for Human BCMA/TNFRSF17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Homozygous BCMA gene deletion in response to anti-BCMA CAR T cells in a patient with multiple myeloma
    Authors: MC Da Vià, O Dietrich, M Truger, P Arampatzi, J Duell, A Heidemeier, X Zhou, S Danhof, S Kraus, M Chatterjee, M Meggendorf, S Twardziok, ME Goebeler, MS Topp, M Hudecek, S Prommersbe, K Hege, S Kaiser, V Fuhr, N Weinhold, A Rosenwald, F Erhard, C Haferlach, H Einsele, KM Kortüm, AE Saliba, L Rasche
    Nature Medicine, 2021-02-22;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Pre-clinical validation of B cell maturation antigen (BCMA) as a target for T cell immunotherapy of multiple myeloma
    Authors: DX Bu, R Singh, EE Choi, M Ruella, S Nunez-Cruz, KG Mansfield, P Bennett, N Barton, Q Wu, J Zhang, Y Wang, L Wei, S Cogan, T Ezell, S Joshi, KJ Latimer, B Granda, WR Tschantz, RM Young, HA Huet, CJ Richardson, MC Milone
    Oncotarget, 2018-05-25;9(40):25764-25780.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2015-07-03;30(1):163-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. gamma -secretase directly sheds the survival receptor BCMA from plasma cells
    Authors: Sarah A. Laurent, Franziska S. Hoffmann, Peer-Hendrik Kuhn, Qingyu Cheng, Yuanyuan Chu, Marc Schmidt-Supprian et al.
    Nature Communications
  5. B-cell Maturation Antigen is a Promising Target for Adoptive T-cell Therapy of Multiple Myeloma.
    Authors: Carpenter, Robert O, Evbuomwan, Moses O, Pittaluga, Stefania, Rose, Jeremy J, Raffeld, Mark, Yang, Shicheng, Gress, Ronald E, Hakim, Frances, Kochenderfer, James N
    Clin Cancer Res, 2013-01-23;19(0):2048-60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Aberrant expression of BAFF by B lymphocytes infiltrating the salivary glands of patients with primary Sjögren's syndrome.
    Authors: Daridon C, Devauchelle V, Hutin P, Le Berre R, Martins-Carvalho C, Bendaoud B, Dueymes M, Saraux A, Youinou P, Pers JO
    Arthritis Rheum., 2007-04-01;56(4):1134-44.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Role of B-cell-activating factor in adhesion and growth of human multiple myeloma cells in the bone marrow microenvironment.
    Authors: Tai YT, Li XF, Breitkreutz I, Song W, Neri P, Catley L, Podar K, Hideshima T, Chauhan D, Raje N, Schlossman R, Richardson P, Munshi NC, Anderson KC
    Cancer Res., 2006-07-01;66(13):6675-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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