Human BCMA/TNFRSF17 Antibody

Name: Human BCMA/TNFRSF17 Antibody
Brand: R&D Systems
SKU: MAB193
Rating: 4 (4 reviews)

Catalog #: MAB193
2 Citations 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB193-SP
MAB193-500
MAB193-100

All BCMA/TNFRSF17 Antibodies

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (4)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BCMA/TNFRSF17 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BCMA in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows no cross‑reactivity with recombinant mouse (rm) BCMA or rmTACI.

Source

Monoclonal Rat IgG_2A Clone # 335004

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BCMA
Met1-Ala54
Accession # Q02223

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.2 - 0.8 μg/mL of this antibody will block 50% of the binding of 500 ng/mL of recombinant human APRIL to immobilized recombinant human BCMA coated at 1 μg/mL (100 μL/well). At 5 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

Madry, C. et al. (1998) Int. Immunol. 10:1693.
Gras, M. et al. (1995) Int. Immunol. 7:1093.
Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
Marsters, S. et al. (2000) Curr. Biol. 10:785.
Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Alternate Names

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human BCMA/TNFRSF17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The effects of BAFF and APRIL signaling on non-small cell lung cancer cell proliferation and invasiveness
Authors: M Warakomska, M Tynecka, D Lemancewic, K Grubczak, J Dzieciol, M Moniuszko, A Eljaszewic, L Bolkun
Oncology Letters, 2021-08-10;22(4):728.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Arrayed molecular barcoding identifies TNFSF13 as a positive regulator of acute myeloid leukemia-initiating cells
Authors: M Chapellier, P Peña-Martí, R Ramakrishn, M Eriksson, M Safaee-Tal, C Orsmark-Pi, H Lilljebjör, C Högberg, A Hagström-A, T Fioretos, J Larsson, M Järås
Haematologica, 2019-02-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization