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Human BCAM Antibody

Catalog #: MAB148
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB148
MAB148-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BCAM Antibody Summary

Species Reactivity
Human
Specificity
Detects human BCAM in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhEpCAM, recombinant mouse (rm) MAdCAM, rhMCAM, rhNCAM-L1, rmOCAM, or rmTROP-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 87202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BCAM
Glu32-Ala547
Accession # P50895
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BCAM Fc Chimera (Catalog # 148-BC) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAM

Basal-Cell Adhesion Molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily (IgSF) protein that differ in the length of their cytoplasmic tails. BCAM cDNA encodes a 628 amino acid (aa) precursor protein with a putative 31 aa signal peptide, a 597 aa extracellular domain containing three C2 type and two V-type Ig like domains, a 21 aa transmembrane domain, and a 19 aa cytoplasmic domain. Compared to the 40 aa cytoplasmic domain present in LU, the BCAM cytoplasmic tail lacks the putative Src homology 3 (SH3) binding site that may be involved in mediating intracellular signaling. BCAM/LU has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM/LU in normal tissues is higher in fetal versus adult tissues. BCAM/LU expression is also upregulated in sickle cell disease red blood cells, in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM/LU has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation.

References
  1. Campbell, I.G. et al. (1994) Cancer Research 54:5761.
  2. Parsons, S.F. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5496.
  3. Udani, M. et al. (1998) J. Clin. Invest. 101:2550.
  4. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.
Long Name
Basal Cell Adhesion Molecule
Entrez Gene IDs
4059 (Human); 57278 (Mouse)
Alternate Names
antigen identified by monoclonal F8; Auberger B antigen; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule; B-CAM cell surface glycoprotein; BCAM; B-cell adhesion molecule; CD239 antigen; CD239; F8/G253 antigen; glycoprotein 95kDa; LUAU; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; MSK19

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Citations for Human BCAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. JAK2V617F activates Lu/BCAM-mediated red cell adhesion in polycythemia vera through an EpoR-independent Rap1/Akt pathway.
    Authors: De Grandis, Maria, Cambot, Marie, Wautier, Marie-Pa, Cassinat, Bruno, Chomienne, Christin, Colin, Yves, Wautier, Jean-Luc, Le Van Kim, Caroline, El Nemer, Wassim
    Blood, 2012-11-16;121(4):658-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin alpha5.
    Authors: Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M
    PLoS ONE, 2011-08-12;6(8):e23329.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Epitope Mapping

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