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Human ATR Antibody

Catalog #: AF4717
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4717
AF4717-SP
Detection of Human ATR by Western Blot.
2 Images
Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ATR Antibody Summary

Species Reactivity
Human
Specificity
Detects human ATR in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ATR
Glu2405-Met2644
Accession # Q13535
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ATR antibody by Western Blot. View Larger

Detection of Human ATR by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ATR at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry ATR antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

ATR in Human Ovarian Cancer Tissue. ATR was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATR

ATR (Ataxia telangiectasia and Rad 3-related) is a member of the phosphatidylinositol kinase-related kinase (PIKK) family of protein kinases. ATR is a large protein kinase (~300 kDa) that functions in the response to genotoxic stress, DNA recombination, and cell cycle control.

Long Name
Ataxia Telangiectasia and Rad 3-related
Entrez Gene IDs
545 (Human); 245000 (Mouse); 685055 (Rat)
Alternate Names
ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR; EC 2.7.11.1; FRAP-related protein 1; FRP1; FRP1FRAP-related protein-1; MEC1; protein kinase ATR; Rad3 related protein; SCKL1; SCKLMEC1, mitosis entry checkpoint 1, homolog; serine/threonine-protein kinase ATR

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Citations for Human ATR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Aberrantly Expressed Timeless Regulates Cell Proliferation and Cisplatin Efficacy in Cervical Cancer
    Authors: Jinhua Zhou, Yinghui Zhang, Xinwei Zou, Lingling Kuai, Li Wang, Juan Wang et al.
    Human Gene Therapy
  2. Time-resolved analysis of DNA-protein interactions in living cells by UV laser pulses
    Authors: A Nebbioso, R Benedetti, M Conte, V Carafa, F De Bellis, J Shaik, F Matarese, B Della Vent, F Gesuele, R Velotta, JHA Martens, HG Stunnenber, C Altucci, L Altucci
    Sci Rep, 2017-09-15;7(1):11725.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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