Human ATG4B Antibody

Catalog #: MAB5279 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5279
MAB5279-SP

All ATG4B Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ATG4B Antibody Summary

Species Reactivity

Human

Specificity

Detects human ATG4B/Apg4b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human ATG4A is observed.

Source

Monoclonal Mouse IgG_2B Clone # 515803

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4B/Apg4b
Asp2-Leu393 (predicted)
Accession # Q9Y4P1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human ATG4B

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant Human ATG4B, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATG4B

Autophagy related protein 4 homolog B (ATG4B), also known as autophagin 1 and Apg4b, is a cytosolic cysteine protease that is involved in autophagy, a biological process in which endogenous protein and damaged organelles are destroyed. Human ATG4B shares 98% and 95% amino acid sequence identity with chimpanzee and mouse ATG4B, respectively.

Long Name

ATG4 Autophagy Related 4 Homolog B

Entrez Gene IDs

23192 (Human); 66615 (Mouse); 316640 (Rat)

Alternate Names

APG4 autophagy 4 homolog B (S. cerevisiae); APG4 autophagy 4 homolog B; Apg4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; AUTL1; AUTL1MGC1353; AUT-like 1 cysteine endopeptidase; autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; cysteine protease ATG4B; DKFZp586D1822; EC 3.4.22; EC 3.4.22.-; hAPG4B; KIAA0943