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Human Amyloid beta (aa1-40) ELISA Kit - Quantikine

Catalog #: DAB140B
18 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DAB140B
Control Products Available
QC216: Quantikine Immunoassay Control Set 1067 Human Amyloid beta
Human Amyloid beta (aa 1-40) ELISA Standard Curve
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Citations (18)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Amyloid beta (aa1-40) Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity
8.17 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
Specificity
Natural human Amyloid beta (aa1-40).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine® Human Amyloid beta (aa1-40) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-40) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-40) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta (aa1-40) showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-40).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 58.9 264 556 56.3 247 518
Standard Deviation 4.23 5.76 11.3 4.31 22.4 30.5
CV% 7.2 2.2 2 7.7 9.1 5.9

Recovery

The recovery of human Amyloid beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 95-105
Cell Lysis Buffer (n=2) 99 91-110
Cerebrospinal Fluid (n=4) 104 96-113

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
Human Amyloid beta (aa 1-40) ELISA Linearity
Human Amyloid beta (aa 1-40) ELISA Linearity

Scientific Data

N/A Human Amyloid beta (aa 1-40) ELISA Standard Curve View Larger

Human Amyloid beta (aa 1-40) ELISA Standard Curve

N/A Human Amyloid beta (aa 1-40) ELISA Standard Curve View Larger

Human Amyloid beta (aa 1-40) ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Amyloid beta

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Entrez Gene IDs:
351 (Human); 11820 (Mouse)
Alternate Names:
Abeta; Amyloid beta; beta-Amyloid Peptide
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Amyloid beta (aa1-40) Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Altered ubiquitin signaling induces Alzheimer's disease-like hallmarks in a three-dimensional human neural cell culture model
    Authors: Maniv, I;Sarji, M;Bdarneh, A;Feldman, A;Ankawa, R;Koren, E;Magid-Gold, I;Reis, N;Soteriou, D;Salomon-Zimri, S;Lavy, T;Kesselman, E;Koifman, N;Kurz, T;Kleifeld, O;Michaelson, D;van Leeuwen, FW;Verheijen, BM;Fuchs, Y;Glickman, MH;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
  2. MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice
    Authors: Ge, J;Xue, Z;Shu, S;Yu, L;Qin, R;Tao, W;Liu, P;Dong, X;Lan, Z;Bao, X;Ye, L;Xu, Y;Zhu, X;
    JCI insight
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Machine-learning-based analysis of the sensitivity and specificity on lipid-lowering effect of one-month-administered statins
    Authors: H Liu, R Jiao, L Wang, F Feng, X Zhao, J Yang
    Medicine, 2023-03-03;102(9):e33139.
    Species: Human
    Sample Types: Serum
  4. Human PSEN1 Mutant Glia Improve Spatial Learning and Memory in Aged Mice
    Authors: H Jäntti, M Oksanen, P Kettunen, S Manta, L Mouledous, H Koivisto, J Ruuth, K Trontti, H Dhungana, M Keuters, I Weert, M Koskuvi, I Hovatta, AM Linden, C Rampon, T Malm, H Tanila, J Koistinaho, T Rolova
    Cells, 2022-12-18;11(24):.
    Species: Human
    Sample Types: Tissue Homogenates
  5. An in vitro workflow of neuron-laden agarose-laminin hydrogel for studying small molecule-induced amyloidogenic condition
    Authors: P Namchaiw, P Bunreangsr, P Eiamcharoe, S Eiamboonse, R P Poo-Arpo
    PLoS ONE, 2022-08-26;17(8):e0273458.
    Species: Human
    Sample Types: Cell Lysates
  6. Blood circulating miR-28-5p and let-7d-5p associate with premature ageing in Down syndrome
    Authors: C Morsiani, MG Bacalini, S Collura, M Moreno-Vil, N Breusing, A Bürkle, T Grune, C Franceschi, M De Eguileo, M Capri
    Mechanisms of Ageing and Development, 2022-06-30;206(0):111691.
    Species: Human
    Sample Types: Plasma
  7. Recurrent moderate hypoglycemia accelerates the progression of cognitive deficits through impairment of TRPC6/GLUT3 pathway in diabetic APP/PS1 mice
    Authors: C He, Q Li, Y Cui, P Gao, W Shu, Q Zhou, L Wang, L Li, Z Lu, Y Zhao, H Ma, X Chen, H Jia, H Zheng, G Yang, D Liu, M Tepel, Z Zhu
    JCI Insight, 2022-03-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Abeta peptides disrupt the barrier integrity and glucose metabolism of human induced pluripotent stem cell-derived brain microvascular endothelial cells
    Authors: S Raut, R Patel, I Pervaiz, AJ Al-Ahmad
    Neurotoxicology, 2022-01-19;89(0):110-120.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Microglial Calhm2 regulates neuroinflammation and contributes to Alzheimer's disease pathology
    Authors: J Cheng, Y Dong, J Ma, R Pan, Y Liao, X Kong, X Li, S Li, P Chen, L Wang, Y Yu, Z Yuan
    Science Advances, 2021-08-25;7(35):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Regulation of beta-amyloid production in neurons by astrocyte-derived cholesterol
    Authors: H Wang, JA Kulas, C Wang, DM Holtzman, HA Ferris, SB Hansen
    Proceedings of the National Academy of Sciences of the United States of America, 2021-08-17;118(33):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. APOE2 mitigates disease-related phenotypes in an isogenic hiPSC-based model of Alzheimer's disease
    Authors: N Brookhouse, S Raman, C Frisch, G Srinivasan, DA Brafman
    Molecular Psychiatry, 2021-04-09;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. The pleiotropic beneficial intervention of olive oil intake on the Alzheimer's disease onset via fibrinolytic system
    Authors: EE Tzekaki, M Tsolaki, &alphaA Pantazaki, G Geromichal, E Lazarou, M Kozori, Z Sinakos
    Experimental gerontology, 2021-04-06;150(0):111344.
    Species: Human
    Sample Types: Serum
  13. Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Authors: IE András, M Garcia-Con, C Yanick, P Perez, B Sewell, L Durand, M Toborek
    Mol Brain, 2020-02-17;13(1):21.
    Species: Human
    Sample Types: Vesicles
  14. Urolithin A attenuates memory impairment and neuroinflammation in APP/PS1 mice
    Authors: Z Gong, J Huang, B Xu, Z Ou, L Zhang, X Lin, X Ye, X Kong, D Long, X Sun, X He, L Xu, Q Li, A Xuan
    J Neuroinflammation, 2019-03-14;16(1):62.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. The neuroprotective effect of deep brain stimulation at nucleus basalis of Meynert in transgenic mice with Alzheimer's disease
    Authors: C Huang, H Chu, Y Ma, Z Zhou, C Dai, X Huang, L Fang, Q Ao, D Huang
    Brain Stimul, 2018-08-27;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
  16. The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro
    Authors: P Liu, X Chen, H Zhou, L Wang, Z Zhang, X Ren, F Zhu, Y Guo, X Huang, J Liu, PS Spencer, X Yang
    Oxid Med Cell Longev, 2018-07-02;2018(0):2025914.
    Species: Mouse
    Sample Types: Cell Lysates
  17. HDAC3 negatively regulates spatial memory in a mouse model of Alzheimer's disease
    Authors: X Zhu, S Wang, L Yu, J Jin, X Ye, Y Liu, Y Xu
    Aging Cell, 2017-08-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Folic acid supplementation improves cognitive function by reducing the levels of peripheral inflammatory cytokines in elderly Chinese subjects with MCI
    Sci Rep, 2016-11-23;6(0):37486.
    Species: Human
    Sample Types: Serum

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