Human Aminopeptidase P2/XPNPEP2 Antibody

Catalog # Availability Size / Price Qty
MAB24901
MAB24901-SP
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Human Aminopeptidase P2/XPNPEP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Aminopeptidase P2/XPNPEP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human XPNEP1 or recombinant mouse XPNEP2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 391131
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase P2/XPNPEP2
His22-Ala650
Accession # AAB96394
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Aminopeptidase P2/XPNPEP2 (Catalog # 2490-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase P2/XPNPEP2 (Catalog # 2490-ZN), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Aminopeptidase P2/XPNPEP2 (2 µg/mL, Catalog # 2490‑ZN) cleavage of the fluorogenic peptide substrate Lys(Abz)-PP-pNA (0.05 mM). The Neutralization Dose (ND50) is typically 1.5 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase P2/XPNPEP2

The human XPNPEP2 gene encodes Aminopeptidase P2 (APP2), which is also known as X-prolyl Aminopeptidase 2 or membrane bound Aminopeptidase P (1‑4). It is a member of the M24 family of metalloproteases, which also contains methionine Aminopeptidases, X-Pro dipeptidase, Aminopeptidase P1, Aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (5). Mammalian APP2 are predicted to be GPI-anchored membrane proteases and their biological functions have been reviewed (6). Human APP2 is widely expressed in many adult tissues with the highest levels in the kidney (7).

References
  1. Venema, R.C. et al. (1997) Biochim. Biophys. Acta 1354:45.
  2. Sprinkle, T.J. et al. (1998) Genomics 50:114.
  3. Cottrell, G.S. et al. (1998) Biochem. Soc. Trans. 26:S248.
  4. Prueitt, R.L. et al. (2000) Cytogenet. Cell Genet. 89:44.
  5. Barrett, A.J. et al. (2004) Handbook of Proteolytic Enzymes, Elsevier Academic Press, San Diego.
  6. Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.) p. 934, Elsevier Academic Press, San Diego.
  7. Ersahin C. et al. (2005) Arch. Biochem. Biophys. 435:303.
Entrez Gene IDs
7512 (Human); 170745 (Mouse)
Alternate Names
Aminoacylproline aminopeptidase; Aminopeptidase P2; APP2; EC 3.4.11.9; mAmP; Membrane-bound aminopeptidase P; Membrane-bound AmP; Membrane-bound APP; xaa-Pro aminopeptidase 2; XPNPEP2; X-Pro aminopeptidase 2; X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound

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Product Specific Notices

The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for non-commercial research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This Product is manufactured under a license to U.S. Patent Nos. 6,399,349 and 7,273,718. Any party desiring rights under this patent should contact Ryogen LLC, Montebello Park, 75 Montebello Road, Suffern, NY 10901.

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