Human Aldo-keto Reductase 1C3/AKR1C3 Antibody

Catalog #: AF7678 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7678
AF7678-SP

Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Western Blot.

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All Aldo-keto Reductase 1C3/AKR1C3 Antibodies

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Human Aldo-keto Reductase 1C3/AKR1C3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Aldo-keto Reductase 1C3/AKR1C3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) AKR1C1 is observed, and approximately 25% cross-reactivity with rhAKR1C4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Aldo-keto Reductase 1C3/AKR1C3
Met1-Tyr323
Accession # P42330

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Western Blot. Western blot shows lysates of Huh-7 human hepatoma cell line, A549 human lung carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aldo-keto Reductase 1C3/AKR1C3

AKR1C3 (Aldo-Keto Reductase family 1 member C3; also 17-beta HSD 5, prostaglandin F synthase/PGFS and 3-alpha HSD type 2) is a 35-36 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. It is expressed by multiple cell types, including prostate epithelium, T cells, and hepatocytes. AKR1C3 generates testosterone and progesterone, catalyzes the conversion of aldehydes and ketones into alcohols, and the mediates the reduction of prostaglandin D2 into PGF2. Human AKR1C3 is 323 amino acids (aa) in length. There are three potential isoform variants. One contains an alternative start site at Met120, a second shows a five aa substitution for aa 1-28, and a third possesses a 15 aa substitution for aa 124-323. Full-length human AKR1C3 shares 73% aa sequence identity with mouse AKR1C3.

Long Name

Aldo-keto Reductase Family 1, Member C3

Entrez Gene IDs

8644 (Human); 105349 (Mouse); 171516 (Rat)

Alternate Names

17-beta-hydroxysteroid dehydrogenase type 5; 3-alpha-HSD type 2; 3-alpha-hydroxysteroid dehydrogenase type 2; AKR1C3; Aldo-keto Reductase 1C3; aldo-keto reductase family 1 member C3; aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II); AldoketoReductase 1C3; Chlordecone reductase homolog HAKRb; DD3; DD-3; DD317-beta-HSD 5; DDH1; DDX; Dihydrodiol dehydrogenase 3; Dihydrodiol dehydrogenase type I; dihydrodiol dehydrogenase X; EC 1.1.1; EC 1.1.1.112,3-alpha-HSD type II, brain; EC 1.1.1.188; EC 1.1.1.213; EC 1.1.1.63; EC 1.1.1.64; EC 1.3.1.20; HA1753; HAKRB; HAKRBtype IIb 3-alpha hydroxysteroid dehydrogenase; HAKRe; HSD17B5; hydroxysteroid (17-beta) dehydrogenase 5; Indanol dehydrogenase; KIAA0119hluPGFS; PGFS; Prostaglandin F synthase; Testosterone 17-beta-dehydrogenase 5; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase