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Human AgRP/ART Biotinylated Antibody

Catalog #: BAF704 Datasheet / COA / SDS
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BAF704
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Human AgRP/ART Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human AgRP/ART in ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse AgRP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human AgRP/ART
Ala21-Thr132
Accession # O00253
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human AgRP/ART (Catalog # 704-AG)

Human AgRP/ART Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human AgRP/ART Antibody (Catalog # MAB8312)

Standard: Recombinant Human AgRP Protein (Catalog # 704-AG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AgRP/ART

Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuropeptide that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) on MC-3 and MC-4 receptors (1 - 4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature human AgRP is a 112 amino acid (aa) peptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Human AgRP shares 82% and 72% aa sequence identity with mouse and rat AgRP, respectively. It shares 32% aa sequence identity with Agouti. As in the case of Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones in a mechanism that does not involve melanocortin receptors (14).

References
  1. Martin, N.M. et al. (2006) Peptides 27:333.
  2. Fan, W. et al. (1997) Nature 385:165.
  3. Ollmann, M.M. et al. (1997) Science 278:135.
  4. Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
  5. Shutter, J.R. et al. (1997) Genes Develop. 11:593.
  6. Kiefer, L.L. et al. (1998) Biochemistry 37:991.
  7. Jackson, P.J. et al. (2002) Biochemistry 41:7565.
  8. Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
  9. Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
  10. Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
  11. Small, C.K. et al. (2001) Diabetes 50:248.
  12. Vink, T. et al. (2001) Mol. Psychiatry 6:325.
  13. Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
  14. Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265 - 266:108.
Long Name
Agouti-related Protein
Entrez Gene IDs
181 (Human); 11604 (Mouse)
Alternate Names
agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2

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