Human Adrenomedullin R/ADMR Antibody

Catalog #: MAB10293 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10293-100
MAB10293-SP

Detection of Adrenomedullin R/ADMR in HEK293 Human Cell Line Transfected with Human Adrenomedullin R/ADMR and eGFP by Flow Cytometry.

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Human Adrenomedullin R/ADMR Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adrenomedullin R/ADMR in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 528563

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Adrenomedullin R/ADMR
Accession # O15218

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

5-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of Adrenomedullin R/ADMR antibody in HEK293 Human Cell Line Transfected with Human Adrenomedullin R/ADMR and eGFP antibody by Flow Cytometry.

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Detection of Adrenomedullin R/ADMR in HEK293 Human Cell Line Transfected with Human Adrenomedullin R/ADMR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Adrenomedullin R/ADMR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adrenomedullin R/ADMR Monoclonal Antibody (Catalog # MAB10293) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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Adrenomedullin R/ADMR in Human Spleen Tissue. Adrenomedullin R/ADMR was detected in immersion fixed paraffin-embedded sections of human spleen tissue using Mouse Anti-Human Adrenomedullin R/ADMR Monoclonal Antibody (Catalog # MAB10293) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cell in sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adrenomedullin R/ADMR

ADMR (GPR182) is a seven transmembrane orphan receptor originally thought to be a receptor for adrenomedullin. ADMR is highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver. Expression is in endothelial cells along the vascular tree.

Long Name

Adrenomedullin Receptor

Entrez Gene IDs

11318 (Human); 11536 (Mouse); 29307 (Rat)

Alternate Names

7TMR; ADMR; Adrenomedullin R; adrenomedullin receptor; AdrenomedullinR; AMR; AM-R; G protein-coupled receptor 182,7TMR; G10D; GAMRH; Gpcr22; GPR182; G-protein coupled receptor 182; hrhAMR; MB10; MGC34399; NOW