Human Adrenomedullin/ADM Antibody

Catalog #: MAB61081 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB61081-100
MAB61081-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adrenomedullin/ADM Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adrenomedullin/ADM in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1003019

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Adrenomedullin/ADM synthetic peptide
Accession # P35318

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

Adrenomedullin/ADM antibody in Human Kidney.

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Adrenomedullin/ADM in Human Kidney. Adrenomedullin/ADM was detected in immersion fixed sections of human kidney tissue using Mouse Anti-Human Adrenomedullin/ADM Monoclonal Antibody (Catalog # MAB61081) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and secreted molecule. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adrenomedullin/ADM

Adrenomedullin (ADM) is a secreted, monomeric, 6 kDa member of the adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. Adrenomedullin has multiple functions including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The human Adrenomedullin preproprecursor is 185 amino acids (aa) in length. It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-92), the Adrenomedullin precursor (amidation is required for maturation) (aa 95-147), and a C-terminal propeptide (aa 148-185). The Adrenomedullin precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated Adrenomedullin (aa 95-146). Depending upon the tissue, truncated forms of Adrenomedullin likely also occur, including variants spanning aa 120-146 and 128-146. Over aa 22-147, the human Adrenomedullin proprecursor shares 70% aa identity with mouse Adrenomedullin proprecursor.

Entrez Gene IDs

133 (Human); 11535 (Mouse); 25026 (Rat)

Alternate Names

ADM; Adrenomedullin; AMpreproadrenomedullin