Human Adenosine A2aR/A2bR Antibody

Catalog #: MAB94972 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB94972-100
MAB94972-SP

Detection of Adenosine A2aR in HEK293 Human Cell Line Transfected with Human Adenosine A2aR and eGFP by Flow Cytometry.

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Human Adenosine A2aR/A2bR Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adenosine A2aR and human Adenosine A2bR in flow cytometry.

Source

Monoclonal Mouse IgG2b Clone # 599743

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Adenosine A2aR in HEK293 Human Cell Line Transfected with Human Adenosine A2aR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosene A2aR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Adenosine A2bR in HEK293 Human Cell Line Transfected with Human Adenosine A2bR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Adenosene A2bR and eGFP was stained with (A) Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) or (B) isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Adenosine A2aR/A2bR

Adenosine is a ubiquitous endogenous molecule that affects almost all aspects of cellular physiology. The four Adenosine G protein-coupled receptors (GPCR) A₁, A_2A, A_2B, and A₃ have been described as key metabolic and immune-checkpoint regulators implicated in the tumor escape from the host immune system becoming both, markers of pathologies, and useful targets for novel drugs. Adenosine receptors A_2A and A_2B (also known as ADORA2A and ADORA2B, respectively) have been also shown to play an important cardio-protective role.

Long Name

Adenosine A2A Receptor/Adenosine A2B Receptor

Alternate Names

Adenosine A2aR/A2bR

Product Datasheets

Product Datasheet

COA

SDS

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