Human Adenosine A2aR/A2bR Alexa Fluor® 750-conjugated Antibody
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Summary
Data Examples
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Human Adenosine A2aR/A2bR Alexa Fluor® 750-conjugated Antibody Summary
Species Reactivity
Human
Specificity
Detects human Adenosine A2aR in direct ELISA.
Source
Monoclonal Mouse IgG2B Clone # 826037
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Adenosine A2aR
Met1-Ser412
Accession # P29274
Met1-Ser412
Accession # P29274
Formulation
Supplied 0.2
mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)
Applications
Recommended Concentration
Sample
Flow Cytometry
Titration recommended for optimal concentration withstarting range of 0.1-1 µg/1 million cells. Sample used for this experiment wasHEK293 cells transfected with HumanADORA-2A and eGFP.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Adenosine A2aR/A2bR
Adenosine is a ubiquitous endogenous molecule that affects almost all aspects of cellular physiology. The four Adenosine G protein-coupled receptors (GPCR) A1, A2A, A2B, and A3 have been described as key metabolic and immune-checkpoint regulators implicated in the tumor escape from the host immune system becoming both, markers of pathologies, and useful targets for novel drugs. Adenosine receptors A2A and A2B (also known as ADORA2A and ADORA2B, respectively) have been also shown to play an important cardio-protective role.
Long Name
Adenosine A2A Receptor/Adenosine A2B Receptor
Alternate Names
Adenosine A2aR/A2bR
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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