Home / ADAM10 / Human ADAM10 Ectodomain Antibody

Human ADAM10 Ectodomain Antibody

Catalog #: AB936
6 Citations Datasheet / COA / SDS
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AB936
ADAM10 in Human Prostate Cancer Tissue.
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Citations (6)
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Human ADAM10 Ectodomain Antibody Summary

Species Reactivity
Human
Specificity
Detects human ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse ADAM10, approximately 5% cross-reactivity with recombinant human (rh) TACE, and less than 1% cross-reactivity with rhADAM8, rhADAM9, rhADAM15, and rhBACE is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM10
Thr214-Glu672
Accession # O14672
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ADAM10 (Catalog # 936-AD)
under non-reducing conditions only
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry ADAM10 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

ADAM10 in Human Prostate Cancer Tissue. ADAM10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 5 µg/mL Goat Anti-Human ADAM10 Ectodomain Polyclonal Antibody (Catalog # AB936) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM10

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, and myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine, and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

References
  1. Rooke, et al. (1996) Science 273:1227.
  2. Pan and Rubin (1997) Cell 90:271.
  3. Qi, et al. (1999) Science 283:91.
  4. Lammich, et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
  5. Rosendahl, et al. (1997) J. Biol. Chem. 272:24588.
  6. Chantry, et al. (1989) J. Biol. Chem. 264:21603.
  7. Millichip, et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
  8. Chantry and Glynn (1990) Biochem. J. 268:245.
  9. Fahrenholz, et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Long Name
A Disintegrin and Metalloprotease-like Domain 10
Entrez Gene IDs
102 (Human); 11487 (Mouse)
Alternate Names
a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease

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Citations for Human ADAM10 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Disintegrin and metalloproteinases (ADAMs) expression in gastroesophageal reflux disease and in esophageal adenocarcinoma
    Authors: T. Kauttu, H. Mustonen, S. Vainionpää, L. Krogerus, I. Ilonen, J. Räsänen et al.
    Clinical and Translational Oncology
  2. Serological immune response against ADAM10 pro-domain is associated with favourable prognosis in stage III colorectal cancer patients
    Authors: SM Álvarez-Fe, M Barbariga, L Cannizzaro, CV Cannistrac, L Hurley, A Zanardi, A Conti, F Sanvito, A Innocenzi, N Pecorelli, M Braga, M Alessio
    Oncotarget, 2016-11-29;7(48):80059-80076.
    Species: Human
    Sample Types: Serum
    Applications: ICC, SDS-Page, Western Blot
  3. ADAM10 controls collagen signaling and cell migration on collagen by shedding the ectodomain of discoidin domain receptor 1 (DDR1)
    Authors: Yasuyuki Shitomi, Ida B. Thøgersen, Noriko Ito, Birgit Leitinger, Jan J. Enghild, Yoshifumi Itoh
    Molecular Biology of the Cell
  4. Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease.
    Authors: Byun K, Bayarsaikhan E, Kim D, Kim CY, Mook-Jung I, Paek SH, Kim SU, Yamamoto T, Won MH, Song BJ, Park YM, Lee B
    PLoS ONE, 2012-05-25;7(5):e37917.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  5. L1 stimulation of human glioma cell motility correlates with FAK activation
    Authors: Muhua Yang, Yupei Li, Kalyani Chilukuri, Owen A. Brady, Magdy I. Boulos, John C. Kappes et al.
    Journal of Neuro-Oncology
  6. Anticancer chemotherapy inhibits MHC class I-related chain a ectodomain shedding by downregulating ADAM10 expression in hepatocellular carcinoma.
    Authors: Kohga K, Takehara T, Tatsumi T, Miyagi T, Ishida H, Ohkawa K, Kanto T, Hiramatsu N, Hayashi N
    Cancer Res., 2009-10-13;69(20):8050-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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