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Human Activin RIIB Antibody

Catalog #: AF339
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF339
AF339-SP
Activin RIIB in Human Placenta.
2 Images
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Activin RIIB Antibody Summary

Species Reactivity
Human
Specificity
Detects human Activin RIIB in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Activin RIIB is observed, and less than 1% cross-reactivity with recombinant human (rh) Activin RIIA, rhActivin RIA, and rhActivin RIB is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Activin RIIB
Ser19-Thr134
Accession # CAA54671
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Activin RIIB Fc Chimera (Catalog # 339-RB)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 30 ng/mL of Recombinant Biotinylated Human/Mouse/Rat Activin A to immobilized Recombinant Human Activin RIIB Fc Chimera (Catalog # 339-RB) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Activin RIIB antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

Activin RIIB in Human Placenta. Activin RIIB was detected in immersion fixed paraffin-embedded sections of human placenta (cross-section of chorionic villi) using 5 µg/mL Goat Anti-Human Activin RIIB Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF339) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Porcine Activin RIIB by Western Blot View Larger

Detection of Porcine Activin RIIB by Western Blot Myostatin signaling in longissimus dorsi muscle of low-birth-weight (LBWT) and normal-birth-weight (NBWT) neonatal pigs. (A): Representative western blots from four pairs of LBWT and NBWT pigs (N, NBWT; L, LBWT). (B–G): Protein abundance of myostain, ALK5 and ActRIIB, and protein abundance phosphorylation of smad2/3. Abundance was normalized to alpha -tubulin and phosphorylation normalized to the corresponding non-phospho-proteins. Results are means ± SE. n = 12. Values with different letters differ significantly (P ≤ 0.05). Image collected and cropped by CiteAb from the following open publication (https://journal.frontiersin.org/article/10.3389/fphys.2017.00482/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIIB

Activin isoforms and other members of the TGF-beta  superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. Seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP‑2/7‑bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

References
  1. Attisano, L. et al. (1996) Mol. and Cell Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.
Long Name
Activin Receptor IIB
Entrez Gene IDs
93 (Human); 11481 (Mouse)
Alternate Names
activin A receptor, type IIB; Activin receptor type IIB; activin receptor type-2B; Activin RIIB; ActivinRIIB; ACTRIIB; ActR-IIB; ACVR2B; EC 2.7.11; EC 2.7.11.30; MGC116908

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Citations for Human Activin RIIB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Downregulated Translation Initiation Signaling Predisposes Low-Birth-Weight Neonatal Pigs to Slower Rates of Muscle Protein Synthesis
    Authors: Y Chen, SR McCauley, SE Johnson, RP Rhoads, SW El-Kadi
    Front Physiol, 2017-07-11;8(0):482.
  2. Circulating activin A during equine gestation and immunolocalization of its receptors system in utero-placental tissues and fetal gonads
    Authors: Pramod DHAKAL, Nobuo TSUNODA, Yasuo NAMBO, Hiroyuki TANIYAMA, Kentaro NAGAOKA, Gen WATANABE et al.
    Journal of Equine Science
  3. Activin receptors regulate the oligodendrocyte lineage in health and disease
    Authors: A Dillenburg, G Ireland, RK Holloway, CL Davies, FL Evans, M Swire, ME Bechler, D Soong, TJ Yuen, GH Su, JC Becher, C Smith, A Williams, VE Miron
    Acta Neuropathol., 2018-02-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination
    Authors: Veronique E. Miron, Amanda Boyd, Jing-Wei Zhao, Tracy J. Yuen, Julia M. Ruckh, Jennifer L. Shadrach et al.
    Nature Neuroscience
  5. Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool.
    Authors: Bristol-Gould SK, Kreeger PK, Selkirk CG, Kilen SM, Cook RW, Kipp JL, Shea LD, Mayo KE, Woodruff TK
    Dev. Biol., 2006-06-17;298(1):132-48.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
    Authors: Maeshima A, Vaughn DA, Choi Y, Nigam SK
    Dev. Biol., 2006-04-27;295(2):473-85.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Activin and inhibin receptor gene expression in the ewe pituitary throughout the oestrous cycle.
    Authors: Fafioffe A, Ethier JF, Fontaine J, JeanPierre E, Taragnat C, Dupont J
    J. Endocrinol., 2004-07-01;182(1):55-68.
    Species: Ovine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Follicle-restricted compartmentalization of transforming growth factor beta superfamily ligands in the feline ovary.
    Authors: Bristol SK, Woodruff TK
    Biol. Reprod., 2003-12-03;70(3):846-59.
    Species: Feline
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Regulation of endocytosis of activin type II receptors by a novel PDZ protein through Ral/Ral-binding protein 1-dependent pathway.
    Authors: Matsuzaki T, Hanai S, Kishi H, Liu Z, Bao Y, Kikuchi A, Tsuchida K, Sugino H
    J. Biol. Chem., 2002-03-06;277(21):19008-18.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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Human Activin RIIB Antibody
By Anonymous on 04/07/2020
Application: ELISA Sample Tested: Human recombinant antibody Species: Human
ELISA Human Activin RIIB Antibody AF339