Human 5-HT7 Antibody

Catalog #: MAB10325 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10325-100
MAB10325-SP

Detection of 5-HT7 in HEK293 Human Cell Line Transfected with Human 5-HT7 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 5-HT7 Antibody Summary

Specificity

Detects human 5-HTR7 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1015322

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing human 5-HTR7
Accession # P34969

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

5-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of 5-HT7 in HEK293 Human Cell Line Transfected with Human 5-HT7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human 5-HT7 and eGFP was stained with (A) Mouse Anti-Human 5-HT7 Monoclonal Antibody (Catalog # MAB10325) or (B) isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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5-HT7 in Human Brain (Hypothalamus). 5-HT7 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human 5-HT7 Monoclonal Antibody (Catalog # MAB10325) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT7

HTR2B is a G-protein coupled receptor for serotonin. The 5HT2 receptors mediate many of the central and peripheral physiologic functions of serotonin. 5HT2B plays a role in the regulation of dopamine and serotonin release, serotonin uptake and in the regulation of extracellular dopamine and serotonin levels. It also plays a role in pulmonary vasoconstriction and regulating cardiac structure and functions. 5HT2B receptors were shown to be expressed in human failing heart and have been strongly implicated in drug induced valvular heart disease.

Long Name

5-Hydroxytryptamine Receptor 7

Entrez Gene IDs

3363 (Human); 15566 (Mouse); 65032 (Rat)

Alternate Names

5HT7; 5-HT7; 5-HT-7; 5-HT75-hydroxytryptamine receptor 7; 5-HT-X; 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled); HTR7; serotonin 5-HT-7 receptor; Serotonin receptor 7